Activities of Ca 2+ -related ion channels during the formation of kidney stones in an infection-induced urolithiasis rat model

Bacterial infection has long been recognized to contribute to struvite urinary stone deposition; however, its contribution to the development of chronic kidney stones has not been extensively investigated. In the present study, we hypothesized another possible method of bacteria contributing to the...

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Published in:American journal of physiology. Renal physiology 2019-11, Vol.317 (5), p.F1342-F1349
Main Authors: Cherng, Juin-Hong, Hsu, Yu-Juei, Liu, Chuan-Chieh, Tang, Shou-Hung, Sartika, Dewi, Chang, Shu-Jen, Fan, Gang-Yi, Wu, Sheng-Tang, Meng, En
Format: Article
Language:English
Subjects:
Animals
Calcium Channels - metabolism
Gene Expression Regulation
Immunity, Innate
Kidney - pathology
Kidney Calculi - metabolism
Proteus Infections - complications
Proteus mirabilis
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Struvite
Urinary Bladder - pathology
Urolithiasis - etiology
Urolithiasis - metabolism
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Summary:Bacterial infection has long been recognized to contribute to struvite urinary stone deposition; however, its contribution to the development of chronic kidney stones has not been extensively investigated. In the present study, we hypothesized another possible method of bacteria contributing to the formation of calcium oxalate (CaOx) that accounts for the biggest part of the kidney stone. Bacteria may play important roles by influencing renal Ca -related ion channel activities, resulting in chronic inflammation of the kidney along with rapid aggregation of stones. We examined the correlation among infection-promoted CaOx kidney stones and alterations in Ca -related ion channels in an animal model with experimentally induced and foreign body infection. After the bladder was infected for 7 days, the data demonstrated that stones were presented and induced severe renal tubular breakage as well as altered levels of monocyte chemoattractant protein-1, cyclooxygenase-2, osteopontin, and transient receptor potential vanilloid member 5 expression, reflecting responses of kidney ion channels. Monocyte chemoattractant protein-1, osteopontin, and transient receptor potential vanilloid member 5 expression was significantly downregulated over time, indicating the chronic inflammation phase of the kidney and accelerated aggregation of CaOx crystals, respectively, whereas cyclooxygenase-2 exhibited no differences. These results indicated that bacterial infection is considerably correlated with an alteration in renal Ca -related ion channels and might support specific and targeted Ca -related ion channel-based therapeutics for urolithiasis and related inflammatory renal damage.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.00199.2019