Serotonergic afferents mediate activity-dependent entrainment of the mouse circadian clock

Serotonergic afferents mediate activity-dependent entrainment of the mouse circadian clock

D. M. Edgar, M. S. Reid and W. C. Dement Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, California 94305, USA. The circadian pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus receives serotonergic afferents from the midbrain raphe nu...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Regulatory, integrative and comparative physiology integrative and comparative physiology, 1997-07, Vol.273 (1), p.265-R269
Main Authors: Edgar, D. M, Reid, M. S, Dement, W. C
Format: Article
Language:eng
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:D. M. Edgar, M. S. Reid and W. C. Dement Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, California 94305, USA. The circadian pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus receives serotonergic afferents from the midbrain raphe nuclei, but the functional role of this projection is unclear. In rodents, locomotor activity increases serotonin content in the SCN, and serotonergic agonists phase shift the circadian clock in a manner closely similar to voluntary bouts of vigorous exercise, suggesting that serotonergic afferents could be part of the activity-dependent entrainment mechanism. We investigated this possibility by selectively lesioning serotonin terminals within and adjacent to the SCN by local microinjection of 5,7-dihydroxytryptamine in mice pretreated with desipramine. This treatment decreased serotonin content 96 +/- 1% and 5-hydroxyindole-3-acetic acid content below levels of detection (nearly 100%) but did not decrease norepinephrine content or neuropeptide Y immunoreactivity in the SCN. These lesions did not alter subsequent running activity levels, yet rendered mice unable to synchronize to a regularly scheduled 2-h wheel running paradigm that entrained sham-lesioned controls. Serotonin afferents are thus necessary for activity-dependent entrainment in the mouse.
ISSN:0363-6119
1522-1490