Intestinal motor disorders associated with cyclical bacterial overgrowth in a rat model of enteritis

The aims of this study were: 1) to obtain an experimental model reproducing the characteristics of chronicity and spontaneous relapses found in inflammatory bowel disease (IBD) and 2) to correlate these changes with intestinal motility and bacteria translocation. For this purpose, two groups of Spra...

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Bibliographic Details
Published in:American journal of physiology: Gastrointestinal and liver physiology 2004-07, Vol.287 (1), p.G58-G64
Main Authors: Porras, Monica, Martín, María Teresa, Soler, Mercè, Vergara, Patri
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Anti-Inflammatory Agents, Non-Steroidal - administration & dosage
Bacteria - growth & development
Bacterial Infections - microbiology
Bacterial Translocation
Cholecystokinin - pharmacology
Chronic Disease
Enteritis - chemically induced
Enteritis - complications
Enteritis - microbiology
Enteritis - pathology
Enzyme Inhibitors - pharmacology
Gastrointestinal Motility
Indomethacin - administration & dosage
Injections, Subcutaneous
Intestinal Diseases - blood
Intestinal Diseases - etiology
Intestinal Diseases - pathology
Intestinal Diseases - physiopathology
Leukocyte Count
Male
Nitroarginine - pharmacology
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha - metabolism
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Summary:The aims of this study were: 1) to obtain an experimental model reproducing the characteristics of chronicity and spontaneous relapses found in inflammatory bowel disease (IBD) and 2) to correlate these changes with intestinal motility and bacteria translocation. For this purpose, two groups of Sprague-Dawley rats were used: a treated group that received two subcutaneous injections of indomethacin (7.5 mg/kg) 48 h apart and a control group that received saline. Blood leukocytes, TNF, and fecal parameters were monitored for 90 days after treatment. In treated rats, a cyclic oscillation of blood leukocytes and TNF concomitant with an inverse correlation of fecal output was observed. Treated rats were then selected either during their highest or lowest blood leukocyte values for motor activity and microbiological evaluation. Controls were obtained in age-matched rats. Rats with high leukocyte levels showed a decrease of motor activity. In contrast, animals with low leukocyte levels presented hypermotility. Bacterial overgrowth accompanied by bacterial translocation was found in the group with high leukocytes, whereas no differences were observed between the control and indomethacin groups during the lowest leukocyte phase. We obtained a model of IBD characterized by a chronic cyclic oscillation of intestinal motility, flora, and inflammatory blood parameters. During the high-leukocyte stage, motor activity decrease is related to bacterial translocation. This phase is followed by a reactive one characterized by hypermotility associated with a decrease in both bacterial growth and leukocytes. However, as in IBD, this reaction seems unable to prevent a return to relapse.
ISSN:0193-1857
1522-1547
DOI:10.1152/ajpgi.00513.2003