Effect of the ovarian hormones on GLUT4 expression and contraction-stimulated glucose uptake

Department of Physiology, The University of Melbourne, Parkville, Victoria 3010, Australia This study examined the roles of the female sex steroids, 17 -estradiol (E 2 ) and progesterone (Prog), on glucose uptake and GLUT4 protein expression. Female Sprague-Dawley rats were either sham operated (C)...

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Bibliographic Details
Published in:American journal of physiology: endocrinology and metabolism 2002-05, Vol.282 (5), p.E1139-E1146
Main Authors: Campbell, S. E, Febbraio, M. A
Format: Article
Language:English
Subjects:
Animals
Deoxyglucose - pharmacokinetics
Estradiol - pharmacology
Female
Glucose - pharmacokinetics
Glucose Transporter Type 4
Glycogen - metabolism
Insulin - blood
Lactic Acid - blood
Monosaccharide Transport Proteins - biosynthesis
Monosaccharide Transport Proteins - metabolism
Muscle Contraction - physiology
Muscle Proteins
Muscle, Skeletal - metabolism
Ovariectomy
Physical Exertion - physiology
Progesterone - pharmacology
Rats
Rats, Sprague-Dawley
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Summary:Department of Physiology, The University of Melbourne, Parkville, Victoria 3010, Australia This study examined the roles of the female sex steroids, 17 -estradiol (E 2 ) and progesterone (Prog), on glucose uptake and GLUT4 protein expression. Female Sprague-Dawley rats were either sham operated (C) or ovariectomized and treated with placebo (O), E 2 (E), Prog (P), or both hormones at physiological doses (P + E) or the same dose of Prog with a high dose of E 2 (P + HiE) via timed-release pellets inserted at the time of surgery, 15 days before metabolic testing. On the morning of day 15 , animals received a 300-µCi injection (ip) of 2-deoxy-[ 14 C]glucose and then either exercised on a motorized treadmill for 30 min at 0.35 m/s or remained sedentary in their cages for the same period. Basal glucose uptake was not different between the treatment groups in either the red or white quadriceps. However, glucose uptake was decreased ( P
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.00184.2001