Evaluation of intramyocellular lipid breakdown during exercise by biochemical assay, NMR spectroscopy, and Oil Red O staining

1 Research Centre for Exercise and Health, Department of Biomedical Kinesiology, and 2 Biomedical Nuclear Magnetic Resonance Unit, Department Medical Diagnostic Sciences, K. U. Leuven, Belgium; and 3 Copenhagen Muscle Research Centre, Institute of Exercise and Sports Sciences, University of Copenhag...

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Published in:American journal of physiology: endocrinology and metabolism 2007-07, Vol.293 (1), p.E428-E434
Main Authors: De Bock, K, Dresselaers, T, Kiens, B, Richter, E. A, Van Hecke, P, Hespel, P
Format: Article
Language:English
Subjects:
Adult
Azo Compounds - pharmacology
Biochemical Phenomena
Biochemistry
Biopsy
Correlation analysis
Cross-Over Studies
Exercise
Exercise - physiology
Humans
Lipid Metabolism
Lipids
Magnetic Resonance Spectroscopy
Male
Muscle Fibers, Skeletal - chemistry
Muscle Fibers, Skeletal - metabolism
Muscular system
NMR
Nuclear magnetic resonance
Staining and Labeling
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Summary:1 Research Centre for Exercise and Health, Department of Biomedical Kinesiology, and 2 Biomedical Nuclear Magnetic Resonance Unit, Department Medical Diagnostic Sciences, K. U. Leuven, Belgium; and 3 Copenhagen Muscle Research Centre, Institute of Exercise and Sports Sciences, University of Copenhagen, Copenhagen, Denmark Submitted 19 February 2007 ; accepted in final form 16 April 2007 The study compared the net decline of intramyocellular lipids (IMCL) during exercise ( n = 18) measured by biochemical assay (BIO) and Oil Red O (ORO) staining on biopsy samples from vastus lateralis muscle and by 1 H-MR spectroscopy (MRS) sampled in an 11 x 11 x 18-mm 3 voxel in the same muscle. IMCL was measured before and after a 2-h cycling bout ( 75% O 2 peak ). ORO and MRS measurements showed substantial IMCL use during exercise of 31 ± 12 and 47 ± 6% of preexercise IMCL content. In contrast, use of BIO for IMCL determination did not reveal an exercise-induced breakdown of IMCL (2 ± 9%, P = 0.29) in young healthy males. Correlations between different measures of exercise-induced IMCL degradation were low. Coefficients were 0.48 for MRS vs. ORO ( P = 0.07) and were even lower for BIO vs. MRS ( r = 0.38, P = 0.13) or ORO ( r = 0.08, P = 0.78). This study demonstrates that different methods to measure IMCL in human muscles can result in different conclusions with regard to exercise-induced IMCL changes. MRS has the advantage that it is noninvasive, however, not fiber type specific and hampered by an at least 30-min delay in measurements after exercise completion and may overestimate IMCL use. BIO is the only quantitative method but is subject to variation when biopsies have different fiber type composition. However, BIO yields lower IMCL breakdown compared with ORO and MRS. ORO has the major advantage that it is fiber type specific, and it therefore provides information that is not available with the other methods. human muscle; exercise; nuclear magnetic resonance Address for reprint requests and other correspondence: P. Hespel, Research Centre for Exercise and Health, FABER-K. U. Leuven, Tervuursevest 101, B-3001 Leuven (Heverlee), Belgium
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.00112.2007