Structural studies on the polysaccharide portion of "A-band" lipopolysaccharide from a mutant (AK1401) of Pseudomonas aeruginosa strain PAO1

AK1401 is a mutant of Pseudomonas aeruginosa strain PAO1 (serotype 05) that does not express O-antigen, but does express "A-band" lipopolysaccharide (LPS). The polysaccharide portion of the A-band LPS (A-PS) from AK1401 was found to consist mainly of D -rhamnose, with smaller amounts of 3-...

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Bibliographic Details
Published in:Canadian journal of chemistry 1991-08, Vol.69 (8), p.1273-1280
Main Authors: Arsenault, Todd L, Hughes, Donald W, MacLean, David B, Szarek, Walter A, Kropinski, Andrew M. B, Lam, Joseph S
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Glycerolipids, phospholipids
Lipids
Other biological molecules
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Summary:AK1401 is a mutant of Pseudomonas aeruginosa strain PAO1 (serotype 05) that does not express O-antigen, but does express "A-band" lipopolysaccharide (LPS). The polysaccharide portion of the A-band LPS (A-PS) from AK1401 was found to consist mainly of D -rhamnose, with smaller amounts of 3-O-methylrhamnose, ribose, mannose, glucose, and a 3-O-methylhexose. 1 H nuclear magnetic resonance spectra of the intact A-PS indicated that the main structural feature was a repeating trisaccharide of α- D -rhamnose having the following structure: The 1 H NMR spectrum of the repeating unit was completely assigned through the use of 2D shift-correlated and relayed coherence transfer NMR spectroscopy. The linkage positions and sequence of residues were found by nuclear Overhauser enhancement difference spectroscopy. Key words: Pseudomonas aeruginosa, lipopolysaccharide, 1 H NMR.
ISSN:0008-4042
1480-3291
DOI:10.1139/v91-190