Effect of electrical stimulation on lipolysis of human white adipocytes

The goal of the present study was to investigate the effect of 30 min of electrical stimulation on the activation of lipolysis in human white adipocytes. Two stimulation protocols (S1, S2) were conducted in vitro on isolated human white adipocytes. Subcutaneous adipose tissue was obtained from femal...

Full description

Saved in:
Bibliographic Details
Published in:Applied physiology, nutrition, and metabolism nutrition, and metabolism, 2011-04, Vol.36 (2), p.271-275
Main Authors: ZIED HAJ HAMIDA, COMTOIS, Alain S, PORTMANN, Michel, BOUCHER, Jean P, SAVARD, Roland
Format: Article
Language:English
Subjects:
adipocytes blancs
Adipocytes, White - metabolism
adipose tissue
Adipose tissues
Adult
Biological and medical sciences
Cells
Electric stimulation
Electric Stimulation Therapy - methods
electrical stimulation
fat cell lipolysis
Female
Fundamental and applied biological sciences. Psychology
Glycerol - metabolism
Health aspects
Humans
In Vitro Techniques
Isoproterenol
Lipids
lipolyse des cellules adipeuses
Lipolysis
Physiological aspects
Physiology
Signal transduction
stimulation électrique
Subcutaneous Fat - metabolism
tissu adipeux
Tissues
Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports
white adipocytes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The goal of the present study was to investigate the effect of 30 min of electrical stimulation on the activation of lipolysis in human white adipocytes. Two stimulation protocols (S1, S2) were conducted in vitro on isolated human white adipocytes. Subcutaneous adipose tissue was obtained from female subjects undergoing abdominal adipose tissue reduction. Adipose tissue of 10 female subjects (mean age, 38.7 ± 9.1 years) and 6 female subjects (mean age, 37.2 ± 11.3 years) was obtained for S1 and S2, respectively. All subjects fasted overnight before tissue removal. The control conditions were a basal and a β-adrenergic stimulation (isoproterenol (ISO), 10 −6  mol·L –1 ) of lipolysis. For S1, the 3 electrostimulation conditions consisted of a monopolar square-wave pulse current for 30 min at intensities of 4, 8, and 20 mA, respectively. In S2, the 2 electrostimulation conditions consisted of a bipolar square-wave alternating current for 30 min at intensities of 4 and 6 mA, respectively. Fat cell lipolysis was measured by quantifying the release of glycerol from adipocytes for 3 trials in each experimental condition. For S1, 4 mA significantly increased lipolysis 1.5 times over basal values (p ≤ 0.01), 8 mA and 20 mA did not increase lipolysis significantly, and no significant difference (p > 0.05) was found between ISO and 4 mA. For S2, 4 mA (p ≤ 0.05) and 6 mA (p ≤ 0.01) significantly increased lipolysis by 1.8 and 2.3 times above basal, respectively. Our results demonstrate that both monopolar (4 mA) and bipolar (4 and 6 mA) electrical stimulations significantly activated in vitro lipolysis. Our findings suggest the existence of a new lipolytic pathway that may involve K v channels shown to exist in human white adipocytes.
ISSN:1715-5312
1715-5320
DOI:10.1139/h11-011