Ultrastructure of BHK-21 cells treated with dopamine

The ultrastructure of a suspension or of adhered BHK-21 cells exposed to dopamine has been studied. It was found that ultrastructural modifications were observed mainly in cellular regions enriched in actin, including in intercellular desmosome-like contacts, microvilli, and under the membrane corti...

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Bibliographic Details
Published in:Cell and tissue biology 2010-03, Vol.4 (1), p.96-102
Main Authors: Shubina, V. S., Abramova, M. B., Lavrovskaya, V. P., Pavlik, L. L., Lezhnev, E. I., Moshkov, D. A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Life Sciences
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Summary:The ultrastructure of a suspension or of adhered BHK-21 cells exposed to dopamine has been studied. It was found that ultrastructural modifications were observed mainly in cellular regions enriched in actin, including in intercellular desmosome-like contacts, microvilli, and under the membrane cortical layer. Desmosome-like contacts are hypertrophied with increased electron density and fibrillar bridges that appear in specialized contacts. Microvilli fused with each other and with plasma membrane of neighboring cells or were split up and penetrated by fibrils. The cytoplasm is filled with randomly arranged F-actin filaments and has a blotchy appearance. The effect of dopamine is more pronounced in nonattached cells with undifferentiated actin cytoskeletons than in spread cells with stress fibers. The blockage of the D2 receptor with haloperidol does not affect the ultrastructure of BHK-21 or dopamine induced modifications. The data obtained suggest direct interactions between dopamine and the actin cytoskeleton.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X10010104