Effect of geldanamycin on expression of signal proteins and heat-shock proteins in normal mouse lymphocytes

Effects of geldanamycin (GA), a known inhibitor of the heat shock protein 90 (Hsp90) activity, on the expression of some signal proteins and various heat-shock proteins were studied by Western blot analysis in cultivated spleen lymphocytes isolated from male NMRI mice. It has been revealed that cult...

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Bibliographic Details
Published in:Cell and tissue biology 2008, Vol.2 (4), p.366-372
Main Authors: Novoselova, T. V., Cherenkov, D. A., Khrenov, M. O., Glushkova, O. V., Lunin, S. M., Novoselova, E. G., Fesenko, E. E.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Life Sciences
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Summary:Effects of geldanamycin (GA), a known inhibitor of the heat shock protein 90 (Hsp90) activity, on the expression of some signal proteins and various heat-shock proteins were studied by Western blot analysis in cultivated spleen lymphocytes isolated from male NMRI mice. It has been revealed that cultivating the cells with GA results in a significant decrease in the amount of transcription factor NF-κB, as well as in its phosphorylated form pNF-κB and the protein suppressing its activity, IκB-α. Furthermore, the presence of GA in the cell-cultivation medium produced a significant decrease in the amount of protein kinase SAPK/JNK. Such a GA-produced modification in the signal protein system suggests the participation of GA in the development of a stress response in an animal cell to damaging action. This suggestion was checked on a model of a cell’s stress response to the action of low-intensity laser radiation. It was proven that the Hsp90-binding agent, GA, significantly decreased in vitro the level of the stress response to laser radiation by decreasing the production of heatshock proteins, Hsp70 and Hsp25, both in irradiated isolated lymphocytes and in their intracellular structures. These findings open prospective uses for geldanamycin in various therapies that are accompanied by undesired side effects connected with increased levels of stress response in immune system cells.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X08040044