Ultrarapid Detection of bla KPC1/2-12 from Perirectal and Nasal Swabs by Use of Real-Time PCR

ABSTRACT The novel real-time PCR assay developed as described here was able to detect bla KPC1/2-12 ( bla KPC-1/2 to bla KPC-12 ) from easily available clinical specimens in less than 2 h. The genotypic assay was highly sensitive (100%) and specific (98%). In some cases, it was able to detect bla KP...

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Bibliographic Details
Published in:Journal of clinical microbiology 2012-05, Vol.50 (5), p.1718-1720
Main Authors: Richter, Sara N., Frasson, Ilaria, Biasolo, Maria Angela, Bartolini, Andrea, Cavallaro, Antonietta, Palù, Giorgio
Format: Article
Language:English
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Summary:ABSTRACT The novel real-time PCR assay developed as described here was able to detect bla KPC1/2-12 ( bla KPC-1/2 to bla KPC-12 ) from easily available clinical specimens in less than 2 h. The genotypic assay was highly sensitive (100%) and specific (98%). In some cases, it was able to detect bla KPC 48 h before positive detection by standard phenotypic assay on patients who were monitored daily. The high sensitivity and rapidity of the molecular method make it the method of choice for KPC surveillance and, thus, containment purposes.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00195-12