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Rapid Onset of Ulcerative Typhlocolitis in B6.129P2-IL10 tm1Cgn (IL-10 −/− ) Mice Infected with Helicobacter trogontum Is Associated with Decreased Colonization by Altered Schaedler's Flora
ABSTRACT Infection with Helicobacter trogontum , a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in B6.129P2-IL10 tm1Cgn (interleukin-10 −/− [IL-10 −/− ]) and C57BL/6 (B6) mice. In a first experiment, IL-10 −/− mice naturally infected with Helicobacter rodenti...
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Published in: | Infection and immunity 2006-12, Vol.74 (12), p.6615-6623 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | ABSTRACT
Infection with
Helicobacter trogontum
, a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in B6.129P2-IL10
tm1Cgn
(interleukin-10
−/−
[IL-10
−/−
]) and C57BL/6 (B6) mice. In a first experiment, IL-10
−/−
mice naturally infected with
Helicobacter rodentium
had subclinical typhlocolitis but developed severe diarrhea and loss of body condition with erosive to ulcerative typhlocolitis within 1 to 3 weeks of experimental infection with
H. trogontum
. A second experiment demonstrated that helicobacter-free IL-10
−/−
mice dosed with
H. trogontum
also developed severe clinical signs and typhlocolitis within 2 to 4 weeks, whereas B6 mice colonized with
H. trogontum
were resistant to disease. In a third experiment, using helicobacter-free IL-10
−/−
mice, dosing with
H. trogontum
resulted in acute morbidity and typhlocolitis within 8 days. Acute typhlocolitis was accompanied by signs of sepsis supported by degenerative hemograms and recovery of
Escherichia coli
and
Proteus
spp. from the livers of infected mice. Quantitative PCR data revealed that
H. rodentium
and
H. trogontum
may compete for colonization of the lower bowel, as
H. trogontum
established higher colonization levels in the absence of
H. rodentium
(
P
< 0.003).
H. trogontum
-induced typhlocolitis was also associated with a significant decrease in the levels of colonization by five of eight anaerobes that comprise altered Schaedler's flora (
P
< 0.002). These results demonstrate for the first time that
H. rodentium
infection in IL-10
−/−
mice causes subclinical typhlocolitis and that infection with
H. trogontum
(with or without
H. rodentium
) induces a rapid-onset, erosive to ulcerative typhlocolitis which impacts the normal anaerobic flora of the colon and increases the risk of sepsis. |
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ISSN: | 0019-9567 1098-5522 |
DOI: | 10.1128/IAI.01091-06 |