The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice

Tyrosine nitration of proteins at an extensive level is widely associated with the cognitive pathology induced by amyloid beta peptide (Abeta). However, the precise identity and explicit consequences of protein nitration have scarcely been addressed. In this study, we examined the detectable nitrati...

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Bibliographic Details
Published in:The Journal of pharmacology and experimental therapeutics 2008-10, Vol.327 (1), p.137-147
Main Authors: Alkam, Tursun, Nitta, Atsumi, Mizoguchi, Hiroyuki, Itoh, Akio, Murai, Rina, Nagai, Taku, Yamada, Kiyofumi, Nabeshima, Toshitaka
Format: Article
Language:English
Subjects:
Amyloid beta-Peptides - toxicity
Animals
Cognition Disorders - chemically induced
Cognition Disorders - metabolism
Cognition Disorders - pathology
Cytoskeleton - metabolism
Hippocampus - metabolism
Hippocampus - pathology
Male
Mice
Mice, Inbred ICR
Neurofilament Proteins - metabolism
Nitrates - metabolism
Phosphorylation
Serine - metabolism
Tyrosine - metabolism
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Summary:Tyrosine nitration of proteins at an extensive level is widely associated with the cognitive pathology induced by amyloid beta peptide (Abeta). However, the precise identity and explicit consequences of protein nitration have scarcely been addressed. In this study, we examined the detectable nitration of proteins in the hippocampus of mice with cognitive impairment (day 5) induced by the i.c.v. injection of Abeta(25-35) (day 0). The intensity of the nitration of proteins was inversely associated with the level of recognition memory in mice. The detectable tyrosine nitrations were revealed in proteins with a single size of approximately 70 kDa. The specific nitrated proteins at this size were identified using the liquid chromatography/mass spectrometry/mass spectrometry analysis and immunodetection methods. Intense nitration of the neurofilament light chain (NFL) was observed. The increased nitration of NFL was associated with its serine hyperphosphorylation and weak interaction with the nuclear distribution element-like, a protein essential for the stable assembly of neurofilaments. No changes in cell numbers in the hippocampus were found (day 5) in mice that received Abeta(25-35) injections. These findings suggested that extensive nitration of NFL is associated with the Abeta-induced impairment of recognition memory in mice.
ISSN:0022-3565
1521-0103
DOI:10.1124/jpet.108.141309