Detection of glutamate in the eye by Raman spectroscopy

Raman spectroscopy is used to detect glutamate in the eye. Glutamate, a by-product of nerve cell death, is an indicator of glaucoma and diabetic retinopathy. The Raman spectra of whole porcine eyes and individual components (lens, cornea, vitreous) are measured and characterized. Monosodium glutamat...

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Bibliographic Details
Published in:Journal of Biomedical Optics 2003-04, Vol.8 (2), p.167-172
Main Authors: Katz, Al, Kruger, Erik F, Minko, Glenn, Liu, C. H, Rosen, Richard B, Alfano, Robert R
Format: Article
Language:English
Subjects:
Animals
Cornea - chemistry
Cornea - drug effects
Cornea - metabolism
diabetic retinopathy
Diabetic Retinopathy - chemically induced
Diabetic Retinopathy - metabolism
Eye - chemistry
Eye - drug effects
Eye - metabolism
glaucoma
Glaucoma - chemically induced
Glaucoma - metabolism
glutamate
In Vitro Techniques
Lens, Crystalline - chemistry
Lens, Crystalline - drug effects
Lens, Crystalline - metabolism
Raman spectroscopy
Sodium Glutamate - administration & dosage
Sodium Glutamate - analysis
Sodium Glutamate - chemistry
Sodium Glutamate - metabolism
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
Swine
Tissue Distribution
Vitreous Body - chemistry
Vitreous Body - drug effects
Vitreous Body - metabolism
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Summary:Raman spectroscopy is used to detect glutamate in the eye. Glutamate, a by-product of nerve cell death, is an indicator of glaucoma and diabetic retinopathy. The Raman spectra of whole porcine eyes and individual components (lens, cornea, vitreous) are measured and characterized. Monosodium glutamate is injected into the eyes to simulate disease conditions, and the contribution to the Raman spectrum due to the presence of glutamate is identified. The Raman spectra from the native eye is dominated by vibrational modes from proteins in the lens. An optical system is designed to optimize collection of signal from the vitreous, where the glutamate is located, and reduce the Raman from the lens. Two vibrational fingerprints of monosodium glutamate are detected at 1369 and 1422 cm , although the concentrations are much above physiological concentrations. ©
ISSN:1083-3668
1560-2281
DOI:10.1117/1.1559726