Loading…
Umbilical cord blood CD 34 + stem cells and other mononuclear cell subtypes processed up to 96 h from collection and stored at room temperature maintain a satisfactory functionality for cell therapy
Background and Objectives Umbilical cord blood ( UCB ) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell ( MNC s) subtypes were viable and functional until 96 h after collection, even stored at room temperature. Now, we analyzed the viability and...
Saved in:
Published in: | Vox sanguinis 2015-01, Vol.108 (1), p.72-81 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Background and Objectives
Umbilical cord blood (
UCB
) is a good stem cell source for cell therapy. We recently demonstrated that cord blood mononuclear cell (
MNC
s) subtypes were viable and functional until 96 h after collection, even stored at room temperature. Now, we analyzed the viability and functionality of the cells before and after cryopreservation.
Materials and Methods
Twenty
UCB
units were analyzed at 24 and 96 h after collection, frozen for 6 months, thawed and re‐evaluated.
MNC
s were analyzed by flow cytometry, viability by 7‐
AAD
and clonogenic assays (
CFU
) were performed.
Results
After 96 h of storage, no substantial loss of
MNC
was found (median 7·320 × 10
6
× 6·305 × 10
6
). Percentage and viability
CD
34
+
cells, B‐cell precursors and mesenchymal stem cells were not affected. However, mature B and T lymphocytes as well as granulocytes had a substantial loss.
CFU
growth was observed in all samples. Prefreezing storage of 96 h was associated with a relative loss of colony formation (median 12%). Postthaw, this loss had a median of 49% (24 h samples) to 56% (96 h samples).
Conclusion
The delay of 96 h before
UCB
processing is possible, without a prohibitive impairment of
CD
34
+
loss in number and functionality. |
---|---|
ISSN: | 0042-9007 1423-0410 |
DOI: | 10.1111/vox.12199 |