Performance of different T rypanosoma cruzi antigens in the diagnosis of C hagas disease in patients with A merican cutaneous leishmaniasis from a co‐endemic region in A rgentina

Abstract Objective To determine the ability of recombinant antigens to detect cases of infection with T rypanosoma cruzi among cases of infection with L eishmania spp. by serological methods. Methods Sera from 41 patients infected with L eishmania spp. were evaluated with ELISA using single ( FRA ,...

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Published in:Tropical medicine & international health 2013-09, Vol.18 (9), p.1103-1109
Main Authors: Vega Benedetti, A.F., Cimino, Rubén O., Cajal, Pamela S., Juarez, Marisa Del Valle, Villalpando, Carlos A., Gil, José F., Marcipar, Iván S., Krolewiecki, Alejandro J., Nasser, Julio R.
Format: Article
Language:English
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Summary:Abstract Objective To determine the ability of recombinant antigens to detect cases of infection with T rypanosoma cruzi among cases of infection with L eishmania spp. by serological methods. Methods Sera from 41 patients infected with L eishmania spp. were evaluated with ELISA using single ( FRA , CP 1 and TSSAVI ) or pooled (commercial R ec‐ ELISA ) recombinant proteins or homogenate antigens (commercial H ‐ ELISA ). As there is no gold standard antigen to discriminate C hagas disease from leishmaniasis, the correlation of results between defined antigens and the homogenate was made with K appa I ndex ( KI ), the level of correlation considered being used as a criterion of specificity. Results Single recombinant antigens and R ec‐ ELISA showed good correlation ( KI  > 0.8). A low correlation ( KI   0,8). Une faible corrélation ( KI  
ISSN:1360-2276
1365-3156
DOI:10.1111/tmi.12144