Identification of an H 2 O 2 permeable PIP aquaporin in barley and a serine residue promoting H 2 O 2 transport

A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H O permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H O -transporting aquaporins among 15 barley...

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Bibliographic Details
Published in:Physiologia plantarum 2017-01, Vol.159 (1), p.120-128
Main Authors: Rhee, Jiye, Horie, Tomoaki, Sasano, Shizuka, Nakahara, Yoshiki, Katsuhara, Maki
Format: Article
Language:English
Subjects:
Aquaporins - genetics
Aquaporins - metabolism
Biological Transport
Cell Membrane - metabolism
Gene Expression
Gene Expression Regulation, Plant
Hordeum - cytology
Hordeum - genetics
Hordeum - metabolism
Hydrogen Peroxide - metabolism
Mutagenesis, Site-Directed
Mutation
Organ Specificity
Oryza - cytology
Oryza - genetics
Oryza - metabolism
Plant Epidermis - genetics
Plant Epidermis - metabolism
Plant Leaves - genetics
Plant Leaves - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Plant Roots - metabolism
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Serine - metabolism
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Summary:A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H O permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H O -transporting aquaporins among 15 barley and rice TIPs. Influx of H O into yeast cells expressing HvPIP2;5 was determined with a florescent-dye-based assay. Indirect immunofluorescence indicated that the expression of HvPIP2;5 protein was ubiquitous in root tissues, and was also weakly observed in leaf epidermal cells and cells in the vascular bundle. Point mutated variants of HvPIP2;5 were generated by the site-directed mutagenesis. Growth assays of yeast cells expressing these mutated HvPIP2;5 proteins suggested that Ser-126 in HvPIP2;5 has a large impact on H O transport with a minor influence on the HvPIP2;5-mediated water transport.
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.12508