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Evaluation of recombinant antigens of T rypanosoma cruzi to diagnose infection in naturally infected dogs from Chaco region, Argentina
Summary Dogs are considered the main mammal reservoir of Trypanosoma cruzi in domiciliary environments. Consequently, accurate detection of T. cruzi infection in canine populations is epidemiologically relevant. Here, we analysed the utility of the T. cruzi recombinant antigens FRA , SAPA , CP 1, Ag...
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Published in: | Parasite immunology 2014-12, Vol.36 (12), p.694-699 |
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Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Summary
Dogs are considered the main mammal reservoir of
Trypanosoma cruzi
in domiciliary environments. Consequently, accurate detection of
T. cruzi
infection in canine populations is epidemiologically relevant. Here, we analysed the utility of the
T. cruzi
recombinant antigens
FRA
,
SAPA
,
CP
1, Ag1 and a
SAPA
/
TSSA VI
mixture, in an
ELISA
format. We used a positive control group of sera obtained from 38 dogs from the Chaco region in Argentina with positive homogenate‐
ELISA
reaction, all of them also positive by xenodiagnosis and/or
PCR
. The negative group included 19 dogs from a nonendemic area. Sensitivity, specificity, area under the curve (
AUC
) of the receiver operating charactheristic (
ROC
) curve and Kappa index were obtained to compare the diagnostic efficiency of the tests. The
SAPA
/
TSSA VI
had the highest performance, with a sensitivity of 94·7% and an
AUC ROC
of 0·99 that indicates high accuracy. Among individual antigens,
SAPA
‐
ELISA
yielded the highest sensitivity (86·8%) and
AUC ROC
(0·96), whereas
FRA
‐
ELISA
was the least efficient test (sensitivity = 36·8%;
AUC ROC
= 0·53). Our results showed that the use of
SAPA
/
TSSA VI
in
ELISA
s could be a useful tool to study dogs naturally infected with
T. cruzi
in endemic areas. |
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ISSN: | 0141-9838 1365-3024 |
DOI: | 10.1111/pim.12144 |