Preferential secretion of inducible HSP 70 by vitiligo melanocytes under stress

Summary Inducible HSP 70 ( HSP 70i) chaperones peptides from stressed cells, protecting them from apoptosis. Upon extracellular release, HSP 70i serves an adjuvant function, enhancing immune responses to bound peptides. We questioned whether HSP 70i differentially protects control and vitiligo melan...

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Published in:Pigment cell and melanoma research 2014-03, Vol.27 (2), p.209-220
Main Authors: Mosenson, Jeffrey A., Flood, Kelsey, Klarquist, Jared, Eby, Jonathan M., Koshoffer, Amy, Boissy, Raymond E., Overbeck, Andreas, Tung, Rebecca C., Le Poole, I. Caroline
Format: Article
Language:English
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Summary:Summary Inducible HSP 70 ( HSP 70i) chaperones peptides from stressed cells, protecting them from apoptosis. Upon extracellular release, HSP 70i serves an adjuvant function, enhancing immune responses to bound peptides. We questioned whether HSP 70i differentially protects control and vitiligo melanocytes from stress and subsequent immune responses. We compared expression of HSP 70i in skin samples, evaluated the viability of primary vitiligo and control melanocytes exposed to bleaching phenols, and measured secreted HSP 70i. We determined whether HSP 70i traffics to melanosomes to contact immunogenic proteins by cell fractionation, western blotting, electron microscopy, and confocal microscopy. Viability of vitiligo and control melanocytes was equally affected under stress. However, vitiligo melanocytes secreted increased amounts of HSP 70i in response to MBEH , corroborating with aberrant HSP 70i expression in patient skin. Intracellular HSP 70i colocalized with melanosomes, and more so in response to MBEH in vitiligo melanocytes. Thus, whereas either agent is cytotoxic to melanocytes, MBEH preferentially induces immune responses to melanocytes.
ISSN:1755-1471
1755-148X
DOI:10.1111/pcmr.12208