m CSF 1, a nucleus‐encoded CRM protein required for the processing of many mitochondrial introns, is involved in the biogenesis of respiratory complexes I and IV in A rabidopsis

Summary The coding regions of many mitochondrial genes in plants are interrupted by intervening sequences that are classified as group II introns. Their splicing is essential for the expression of the genes they interrupt and hence for respiratory function, and is facilitated by various protein cofa...

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Published in:The New phytologist 2013-07, Vol.199 (2), p.379-394
Main Authors: Zmudjak, Michal, Colas des Francs‐Small, Catherine, Keren, Ido, Shaya, Felix, Belausov, Eduard, Small, Ian, Ostersetzer‐Biran, Oren
Format: Article
Language:English
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Summary:Summary The coding regions of many mitochondrial genes in plants are interrupted by intervening sequences that are classified as group II introns. Their splicing is essential for the expression of the genes they interrupt and hence for respiratory function, and is facilitated by various protein cofactors. Despite the importance of these cofactors, only a few of them have been characterized. CRS 1‐ Y hb Y domain ( CRM ) is a recently recognized RNA ‐binding domain that is present in several characterized splicing factors in plant chloroplasts. The A rabidopsis genome encodes 16 CRM proteins, but these are largely uncharacterized. Here, we analyzed the intracellular location of one of these hypothetical proteins in A rabidopsis, mitochondrial CAF ‐like splicing factor 1 (m CSF 1; A t4 g31010), and analyzed the growth phenotypes and organellar activities associated with mcsf1 mutants in plants. Our data indicated that mCSF 1 resides within mitochondria and its functions are essential during embryogenesis. Mutant plants with reduced m CSF 1 displayed inhibited germination and retarded growth phenotypes that were tightly associated with reduced complex I and IV activities. Analogously to the functions of plastid‐localized CRM proteins, analysis of the RNA profiles in wildtype and mcsf1 plants showed that m CSF 1 acts in the splicing of many of the group II intron RNA s in A rabidopsis mitochondria.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.12282