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Keratinocyte cell lines derived from severe generalized recessive E pidermolysis B ullosa patients carrying a highly recurrent COL 7A1 homozygous mutation: models to assess cell and gene therapies in vitro and in vivo
Abstract Recessive dystrophic epidermolysis bullosa ( RDEB ) is caused by deficiency of type VII collagen due to COL 7A1 mutations such as c.6527insC, recurrently found in the S panish RDEB population. Assessment of clonal correction–based therapeutic approaches for RDEB requires large expansions of...
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Published in: | Experimental dermatology 2013-09, Vol.22 (9), p.601-603 |
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Main Authors: | , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abstract
Recessive dystrophic epidermolysis bullosa (
RDEB
) is caused by deficiency of type VII collagen due to
COL
7A1
mutations such as c.6527insC, recurrently found in the
S
panish
RDEB
population. Assessment of clonal correction–based therapeutic approaches for
RDEB
requires large expansions of cells, exceeding the replication capacity of human primary keratinocytes. Thus, immortalized
RDEB
cells with enhanced proliferative abilities would be valuable. Using either the
SV
40 large
T
antigen or papillomavirus
HPV
16‐derived E6‐E7 proteins, we immortalized and cloned
RDEB
keratinocytes carrying the c.6527insC mutation. Clones exhibited high proliferative and colony‐forming features. Cytogenetic analysis revealed important differences between
T
antigen‐driven and
E
6‐
E
7‐driven immortalization. Immortalized cells responded to differentiation stimuli and were competent for epidermal regeneration and recapitulation of the blistering
RDEB
phenotype
in vivo
. These features make these cell lines useful to test novel therapeutic approaches including those aimed at editing mutant
COL
7A1
. |
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ISSN: | 0906-6705 1600-0625 |
DOI: | 10.1111/exd.12203 |