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Different sealing materials preventing the microbial leakage into the screw‐retained implant restorations: an in vitro analysis by DNA c heckerboard hybridization
Abstract Objectives The aim of this controlled in vitro study was to identify and quantify up to 38 microbial species penetrating through the screw‐retained implant prostheses with different sealing materials. Material and methods Sixty morse cone implants were restored with single‐unit screw‐retain...
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Published in: | Clinical oral implants research 2017-02, Vol.28 (2), p.242-250 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Abstract
Objectives
The aim of this controlled
in vitro
study was to identify and quantify up to 38 microbial species penetrating through the screw‐retained implant prostheses with different sealing materials.
Material and methods
Sixty morse cone implants were restored with single‐unit screw‐retained prostheses. All the components were randomly divided into five groups (
n
= 12) according to the proposed materials: (1) polytetrafluoroethylene tape+composite resin; (2) polytetrafluoroethylene tape+gutta‐percha; (3) polytetrafluoroethylene tape+light‐polymerized provisional composite; (4) cotton pellet+gutta‐percha; and (5) cotton pellet+light‐polymerized provisional composite. Human saliva was used as contaminant media, and
DNA
checkerboard hybridization was used to identify and quantify microbial species.
Results
Microbial leakage was observed in all groups:
M. salivarium
,
S. pasteuri
,
P. nigrescens
, and
P. melaninogenica
were the species presenting the highest values of genome count, prevalence, and proportion within the groups. The total microbial mean counts (×10
5
, ±
SD
) were as follows: Group 1 (2.81 ± 0.38), Group 2 (3.41 ± 0.38), Group 3 (6.02 ± 1.48), Group 4 (6.40 ± 1.42), and Group 5 (17.45 ± 1.67). Group 5 showed the higher microbial counts (
P
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ISSN: | 0905-7161 1600-0501 |
DOI: | 10.1111/clr.12790 |