High frequency of multidrug‐resistant Staphylococcus aureus with SCC mec type III and Spa types t037 and t631 isolated from burn patients in southwest of Iran

Methicilin resistance Staphylococcus aureus ( MRSA ) infections are the major challenges in hospitals, especially in the burn units. The use of molecular typing methods is essential for tracking the spread of S. aureus infection and epidemiological investigations. The aim of this study was to find t...

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Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2016-03, Vol.124 (3), p.221-228
Main Authors: Parhizgari, Najmeh, Khoramrooz, Seyed Sajjad, Malek Hosseini, Seyed Ali Asghar, Marashifard, Masoud, Yazdanpanah, Mahboobeh, Emaneini, Mohammad, Gharibpour, Farzaneh, Mirzaii, Mehdi, Darban‐Sarokhalil, Davood, Moein, Masoud, Naraki, Mahmood
Format: Article
Language:English
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Summary:Methicilin resistance Staphylococcus aureus ( MRSA ) infections are the major challenges in hospitals, especially in the burn units. The use of molecular typing methods is essential for tracking the spread of S. aureus infection and epidemiological investigations. The aim of this study was to find the profile of the spa types and also the prevalence of each SCC mec type of S. aureus strains in a central burn hospital in southwest of Iran. A total of 81 non‐duplicate S. aureus were isolated from burn patients between April 2011 and February 2012. The susceptibility of the isolates against 13 different antibiotics was tested by disk agar diffusion ( DAD ) method. MRSA strains were identified by amplification of mec A gene. Multiplex‐polymerase chain reaction ( PCR ) technique was used to determine the SCC mec types of MRSA strains and all the S. aureus isolates were typed by spa typing method. Detection of mec A gene showed that 70 (86.4%) of the isolates were MRSA . The highest rate of resistance was observed for penicillin (97.5%) and erythromycin (77.8%). None of the isolates were resistant to vancomycin. Sixty‐seven of the 70 MRSA isolates harbored only SCC mec type III and three untypeable isolates. Five different spa types were detected. The most common spa types were t037 (42.5%) and t631 (34.5%) and were only found in MRSA isolates. Only SCC mec type III was found in burn patients which emphasizes the HA ‐ MRSA origin of these strains. Only five different spa types identified in this study are in accordance with one SCC mec type which indicates that a limited number of bacterial colons are circulated in the burn unit in this hospital.
ISSN:0903-4641
1600-0463
DOI:10.1111/apm.12493