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Cardioprotective Effect of Micro RNA ‐21 in Murine Myocardial Infarction
Summary Introduction To investigate the cardioprotective effect of Micro RNA ‐21 (miR‐21) in murine myocardial infarction ( MI ). Methods Forty C57 BL /6 male mice were divided into sham group, MI group, LV ‐ GFP group, and miR‐21 group. Mice in the MI group, LV ‐ GFP group, and miR‐21 group were su...
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| Published in: | Cardiovascular therapeutics 2015-06, Vol.33 (3), p.109-117 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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Introduction
To investigate the cardioprotective effect of Micro
RNA
‐21 (miR‐21) in murine myocardial infarction (
MI
).
Methods
Forty C57
BL
/6 male mice were divided into sham group,
MI
group,
LV
‐
GFP
group, and miR‐21 group. Mice in the MI group,
LV
‐
GFP
group, and miR‐21 group were subjected to
MI
by left anterior descending artery (
LAD
) ligation, while chest was opened/closed without ligation in sham group. In
MI
group, expression of miR‐21 in the
MI
area and its surrounding areas was detected at 1st, 2nd, and 4th week after experiment. Subsequently, lentivirus expressing miR‐21 and lentivirus that did not express miR‐21 were transfected into mice left ventricular cavity of miR‐21 group and
LV
‐
GFP
group, respectively. Cardiac function,
MI
size, miR‐21 expression, collagen I level, fibronectin content, number of
α
‐
SMA
‐positive cells, number of apoptotic cells, apoptosis‐related factors were compared between the three groups.
Results
Compared with sham group, miR‐21 levels in
MI
group were significantly decreased in the 1st week and 2nd week, but were almost the same in the 4th week. Left ventricular fractional shortening (
LVFS
) and left ventricular ejection fraction (
LVEF
) in the miR‐21 group improved compared to the
LV
‐
GFP
group. In miR‐21 group, myocardial infarct size reduced by 36.9% in comparison with
LV
‐
GFP
group. Compared to sham group, miR‐21 expression in the miR‐21 group and
LV
‐
GFP
group decreased significantly. In the miR‐21 group, collagen I level, fibronectin content and number of
α
‐
SMA
‐positive cells of miR‐21 decreased significantly compared to the
LV
‐
GFP
group. The number of apoptotic cells in the
MI
areas of the miR‐21 group was significantly less than the
LV
‐
GFP
group. Compared with the
LV
‐
GFP
group, Bcl‐2 level and the ratio of Bcl‐2 to Bax were significantly increased, and the levels of Bax and Caspase‐3 decreased.
Conclusions
Our results suggest miR‐21 is an important regulatory molecule in the pathophysiology of
MI
. |
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| ISSN: | 1755-5914 1755-5922 |
| DOI: | 10.1111/1755-5922.12118 |