Upregulation of intraepithelial lymphocyte (IEL) function in the small intestinal mucosa in sepsis

Host defense mechanisms preventing bacterial invasion are particularly important in the gastrointestinal tract, since most gram-negative infections originate from there. Intraepithelial lymphocytes (IEL) seem to play an important role in this immune surveillance of the intestine, although their func...

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Bibliographic Details
Published in:Shock (Augusta, Ga.) Ga.), 2001-12, Vol.16 (6), p.454-458
Main Authors: NÜSSLER, Natascha C, STANGE, Barbara, NUSSLER, Andreas K, SETTMACHER, Utz, LANGREHR, Jan M, NEUHAUS, Peter, HOFFMAN, Rosemary A
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Cell Division
Cytotoxicity, Immunologic
Emergency and intensive care: infection, septic shock
Endotoxemia - immunology
Endotoxemia - pathology
Endotoxemia - physiopathology
Female
In Vitro Techniques
Intensive care medicine
Interferon-gamma - biosynthesis
Intestinal Mucosa - immunology
Intestinal Mucosa - pathology
Intestinal Mucosa - physiopathology
Intestine, Small - immunology
Intestine, Small - pathology
Intestine, Small - physiopathology
Lipopolysaccharides - toxicity
Lymphocyte Subsets - drug effects
Lymphocyte Subsets - immunology
Lymphocyte Subsets - pathology
Lymphocyte Subsets - physiology
Lymphocytes - drug effects
Lymphocytes - immunology
Lymphocytes - pathology
Lymphocytes - physiology
Medical sciences
Mice
Mice, Inbred C57BL
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase Type II
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sepsis - immunology
Sepsis - pathology
Sepsis - physiopathology
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Summary:Host defense mechanisms preventing bacterial invasion are particularly important in the gastrointestinal tract, since most gram-negative infections originate from there. Intraepithelial lymphocytes (IEL) seem to play an important role in this immune surveillance of the intestine, although their function in sepsis is not fully understood. To evaluate the characteristics of IEL in sepsis, C57BL/6 mice received a non-lethal dose of LPS and IEL were harvested at various time points thereafter. Although IEL displayed no phenotypic changes after endotoxemia, they displayed enhanced cytolytic activity and increased proliferation after LPS injection In addition, IEL from septic mice showed enhanced gamma interferon (IFN-gamma) production after LPS administration. The production of IFN-gamma may have induced the increased intestinal NOS-2 mRNA expression which was observed after endotoxemia. In conclusion, endotoxemia leads to functional activation of IEL without phenotypic changes. The activation of IEL and the subsequently increased NOS-2 expression may be important mechanisms in maintaining the mucosal barrier after sublethal LPS challenge.
ISSN:1073-2322
1540-0514
DOI:10.1097/00024382-200116060-00009