Endoglin regulates nitric oxide‐dependent vasodilatation

Endoglin is a membrane glycoprotein that plays an important role in cardiovascular development and angiogenesis. We examined the role of endoglin in the control of vascular tone by measuring nitric oxide (NO)‐dependent vasodilation in haploinsufficient mice (Eng+/−) and their Eng+/+ littermates. The...

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Bibliographic Details
Published in:The FASEB journal 2004-03, Vol.18 (3), p.609-611
Main Authors: Jerkic, Mirjana, Rivas‐Elena, Juan V., Prieto, Marta, Carrón, Rosalia, Sanz‐Rodríguez, Francisco, Pérez‐Barriocanal, Fernando, Rodríguez‐Barbero, Alicia, Bernabéu, Carmelo, López‐Novoa, J.M.
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Acetylcholine - toxicity
Animals
Antigens, CD
Blood Pressure - drug effects
Bradykinin - toxicity
Cell Line
Endoglin
endoglin haploinsufficiency
Endothelial Cells - metabolism
Endothelium, Vascular - metabolism
eNOS expression
Enzyme Induction - physiology
Hypotension - chemically induced
Kidney - metabolism
Lung - metabolism
Mice
Mice, Knockout
Myoblasts - cytology
Myoblasts - metabolism
NG-Nitroarginine Methyl Ester - pharmacology
Nitric Oxide - physiology
Nitric Oxide Donors - pharmacology
Nitric Oxide Synthase - biosynthesis
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Nitroprusside - pharmacology
Receptors, Cell Surface
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - physiology
Transfection
Transforming Growth Factor beta - deficiency
Transforming Growth Factor beta1
Vascular Cell Adhesion Molecule-1 - biosynthesis
Vascular Cell Adhesion Molecule-1 - genetics
Vascular Cell Adhesion Molecule-1 - physiology
vascular tone
Vasoconstrictor Agents - pharmacology
Vasodilation - physiology
Vasodilator Agents - pharmacology
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Summary:Endoglin is a membrane glycoprotein that plays an important role in cardiovascular development and angiogenesis. We examined the role of endoglin in the control of vascular tone by measuring nitric oxide (NO)‐dependent vasodilation in haploinsufficient mice (Eng+/−) and their Eng+/+ littermates. The vasodilatory effect of acetylcholine, bradykinin, and sodium nitroprusside was assessed in anesthetized mice; in isolated, perfused hindlimbs; and in aortic rings. The substantial hypotensive and vasodilatory response induced by acetylcholine and bradykinin in Eng+/+ was markedly reduced in Eng+/− mice. Both kinds of animals had similar responses to sodium nitroprusside, suggesting that the deficient vasodilatory effect is not due to a NO response impairment. Urinary and plasma concentrations of nitrites, a NO metabolite, were lower in Eng+/− than in Eng+/+ mice. The levels of endothelial nitric oxide synthase (eNOS) in kidneys and femoral arteries were about half in Eng+/− than in Eng+/+ mice and were also reduced in primary cultures of aortic endothelial cells from Eng+/− compared with those from Eng+/+ mice. Furthermore, overexpression or suppression of endoglin in cultured cells induced a marked increase or decrease in the protein levels of eNOS, respectively. Thus, our results in vivo and in vitro demonstrate a relationship between endoglin and NO‐dependent vasodilation mediated by the regulation of eNOS expression.
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.03-0197fje