Proteomic Analysis of Calcium Effects on Soybean Root Tip under Flooding and Drought Stresses

Flooding and drought are disadvantageous environmental conditions that induce cytosolic calcium in soybean. To explore the effects of flooding- and drought-induced increases in calcium, a gel-free/label-free proteomic analysis was performed. Cytosolic calcium was decreased by blocking calcium channe...

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Bibliographic Details
Published in:Plant and cell physiology 2017-08, Vol.58 (8), p.1405-1420
Main Authors: Wang, Xin, Komatsu, Setsuko
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Boron Compounds - pharmacology
Calcium - metabolism
Calnexin - genetics
Calnexin - metabolism
Droughts
Endoplasmic Reticulum - metabolism
Endoplasmic Reticulum Stress - genetics
Floods
Gene Expression Regulation, Plant - drug effects
Glycine max - drug effects
Glycine max - metabolism
Glycine max - physiology
Glycolysis - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - physiology
Proteomics - methods
Pyruvate Decarboxylase - metabolism
Stress, Physiological
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Summary:Flooding and drought are disadvantageous environmental conditions that induce cytosolic calcium in soybean. To explore the effects of flooding- and drought-induced increases in calcium, a gel-free/label-free proteomic analysis was performed. Cytosolic calcium was decreased by blocking calcium channels in the endoplasmic reticulum (ER) and plasma membrane under both stresses. Calnexin, protein disulfide isomerase, heat shock proteins and thioredoxin were predominantly affected as the ER proteins in response to calcium, and ER-associated degradation-related proteins of HCP-like superfamily protein were up-regulated under stress exposure and then down-regulated. Glycolysis, fermentation, the tricarboxylic acid cycle and amino acid metabolism were mainly induced as the types of cellular metabolism in response to calcium under both stresses. Pyruvate decarboxylase was increased and decreased under flooding and drought, respectively, and was further decreased by the reduction of cytosolic calcium; however, it was recovered by exogenous calcium under both stresses. Furthermore, pyruvate decarboxylase activity was increased under flooding, but decreased under drought. These results suggest that calcium is involved in protein folding in the ER, and ER-associated degradation might alleviate ER stress during the early stage of both stresses. Furthermore, calcium appears to modify energy metabolism, and pyruvate decarboxylase may be a key enzyme in this process under flooding and drought.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcx078