In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice

Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node in vivo. Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented w...

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Bibliographic Details
Published in:Organogenesis 2019-07, Vol.15 (3), p.85-99
Main Authors: Zhang, Jing, Xu, Yuqiao, Liu, Tao, Min, Jie, Ma, Yu, Song, Yongli, Lu, Jianrong, Mi, Wenjuan, Wang, Yingmei, Li, Hang, Li, Wangzhou, Zhao, Daqing
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipose Tissue - cytology
adipose-derived stromal cells
Animals
Cell Differentiation
Cell Lineage
Cell Proliferation
cytokine
Fibroblast Growth Factor 2 - pharmacology
Humans
Hydrogels - chemistry
hydroxypropyl methyl cellulose
Hypromellose Derivatives - chemistry
Immunohistochemistry
Lymph Nodes - cytology
lymphocyte
Lymphocytes - cytology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Organ Culture Techniques
Phenotype
Research Paper
Stromal Cells - cytology
Tissue Engineering - methods
Transforming Growth Factor beta - pharmacology
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Summary:Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node in vivo. Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented with transforming growth factor β1 (TGF)-β1 and basic fibroblast growth factor (bFGF). The induced hADSCs mixed with 13% (w/v) hydroxypropyl methylcellulose (HPMC) were injected into BALB/c nude mice subcutaneously. Eight weeks later, nodules were found under the injected sites. Histology, immunohistochemistry, and species identification analysis confirmed that the nodules were lymphoid nodes that were derived from the injected hADSCs. Our experiment demonstrated that the hADSCs could differentiate into lymphocyte-like cells and form lymphoid nodes in vivo. TGF-β1 and bFGF might play important roles in the differentiation of hADSCs into lymphocyte-like cells. Our study might present an alternative approach for engineering immune organs and thus offer potential treatment for immunodeficiency diseases.
ISSN:1547-6278
1555-8592
DOI:10.1080/15476278.2019.1656994