DNA groove-binding and acid-base properties of a Ru(II) complex containing anthryl moieties

DNA groove binders have been poorly studied as compared to the intercalators. A novel Ru(II) complex of [Ru(aeip) 2 (Haip)](PF 6 ) 2 {Haip = 2-(9-anthryl)-1H-imidazo[4,5-f][1,10]phenanthroline and aeip = 2-(anthracen-9-yl)-1-ethyl-imidazo[4,5-f][1, 10]phenanthroline} is synthesized and characterized...

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Bibliographic Details
Published in:Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2020-04, Vol.39 (4), p.592-614
Main Authors: Yin, Hong-Ju, Zhang, An-Guo, Gao, Li-Hua, Zhao, Hua, Wang, Ke-Zhi
Format: Article
Language:English
Subjects:
Animals
anthracene
Binding, Competitive
Cattle
Coordination Complexes - chemical synthesis
Coordination Complexes - chemistry
density functional theory
Deoxyribonucleic acid
DNA
DNA - chemistry
Ethidium - chemistry
Ethidium bromide
Hydrogen-Ion Concentration
Magnetic resonance spectroscopy
Mass spectroscopy
Molecular Structure
NMR
Nuclear magnetic resonance
Nucleic Acid Denaturation
Ruthenium
Ruthenium - chemistry
Spectrophotometry
Viscosity
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Summary:DNA groove binders have been poorly studied as compared to the intercalators. A novel Ru(II) complex of [Ru(aeip) 2 (Haip)](PF 6 ) 2 {Haip = 2-(9-anthryl)-1H-imidazo[4,5-f][1,10]phenanthroline and aeip = 2-(anthracen-9-yl)-1-ethyl-imidazo[4,5-f][1, 10]phenanthroline} is synthesized and characterized by elemental analysis, 1 H NMR spectroscopy and mass spectrometry. The complex is evidenced to be a calf-thymus DNA groove binder with a large intrinsic binding constant of 10 6  M −1 order of magnitude as supported by UV-visible absorption spectral titrations, salt effects, DNA competitive binding with ethidium bromide, DNA melting experiment, DNA viscosity measurements and density functional theory calculations. The acid-base properties of the complex studied by UV-Vis spectrophotometric titrations are reported as well.
ISSN:1525-7770
1532-2335
DOI:10.1080/15257770.2019.1669804