The Neuroprotective Adenosine-Activated Signal Transduction Pathway Involves Activation of Phospholipase C

We have demonstrated before that exposure of neuronal cultures to poisoning by iodoacetic acid (IAA) followed by "reperfusion" (IAA-R insult), results in severe cytotoxicity, which could be markedly attenuated by prior activation of the adenosine A 1 receptors. We also have demonstrated th...

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Bibliographic Details
Published in:Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2006-06, Vol.25 (9-11), p.1283-1286
Main Authors: Rogel, A., Bromberg, Y., Sperling, O., Zoref-Shani, E.
Format: Article
Language:English
Subjects:
Adenosine
Adenosine - metabolism
Animals
Brain - embryology
Enzyme Activation
Enzyme Inhibitors - pharmacology
Estrenes - pharmacology
Iodoacetic acid
Iodoacetic Acid - pharmacology
Neurons - metabolism
Neuroprotection
Neuroprotective Agents - pharmacology
Phospholipase C (PLC)
Pyrrolidinones - pharmacology
Rats
Reperfusion Injury
Signal Transduction
Signal transduction pathway
Type C Phospholipases - metabolism
U73122
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Summary:We have demonstrated before that exposure of neuronal cultures to poisoning by iodoacetic acid (IAA) followed by "reperfusion" (IAA-R insult), results in severe cytotoxicity, which could be markedly attenuated by prior activation of the adenosine A 1 receptors. We also have demonstrated that adenosine activates a signal transduction pathway (STP), which involves activation of PKCĪµ and opening of K ATP channels. Here, we provide proof for the involvement also of phospholipase C (PLC) in the neuronal protective adenosine-activated STP. R-PIA, a specific A 1 adenosine receptor agonist, was found to enhance neuronal PLC activity and protect against the IAA-R insult. The PLC inhibitor U73122, abrogated both R-PIA-induced effects. These results demonstrate that activation of PLC is a vital step in the neuronal protective adenosine-induced STP.
ISSN:1525-7770
1532-2335
DOI:10.1080/15257770600890939