Molecular cloning and characterization of a cDNA encoding the Paracoccidioides brasiliensis l35 ribosomal protein1

A 630 bp cDNA encoding an L35 ribosomal protein of Paracoccidioides brasiliensis, designated as Pbl35, was cloned from a yeast expression library. Pbl35 encodes a polypeptide of 125 amino acids, with a predicted molecular mass of 14.5 kDa and a pI of 11.0. The deduced PbL35 shows significant conserv...

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Bibliographic Details
Published in:Medical mycology (Oxford) 2004-06, Vol.42 (3), p.217-221
Main Authors: Jesuino, Rosália S. A., Pereira, Maristela, Felipe, M. Sueli S., Azevedo, Maristella O., Soares, Célia M. A.
Format: Article
Language:English
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Summary:A 630 bp cDNA encoding an L35 ribosomal protein of Paracoccidioides brasiliensis, designated as Pbl35, was cloned from a yeast expression library. Pbl35 encodes a polypeptide of 125 amino acids, with a predicted molecular mass of 14.5 kDa and a pI of 11.0. The deduced PbL35 shows significant conservation in respect to other described ribosomal L35 proteins from eukaryotes and prokaryotes. Motifs of ribosomal proteins are present in PbL35, including a bipartite nuclear localization signal (NLS) that could be related to the protein addressing to the nucleolus for the ribosomal assembly. The mRNA for PbL35, about 700 nucleotides in length, is expressed at a high level in P. brasiliensis. The PbL35 and the deduced amino acid sequence constitute the first description of a ribosomal protein in P. brasiliensis. The cDNA was deposited in GenBank under accession number AF416509.
ISSN:1369-3786
1460-2709
DOI:10.1080/13693780310001597692