Expression of atrial natriuretic peptide in the heart of active and hibernating frog

The expression of atrial natriuretic peptides (ANP) - pro ANP26-92 and αANP - was investigated with immunohistochemical methods in the heart of Rana kl. esculenta by comparing active and hibernating frogs. The distribution of ANP binding sites was studied by cytochemical demonstration of guanylate c...

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Bibliographic Details
Published in:The Italian journal of zoology 2004-01, Vol.71 (sup2), p.69-72
Main Authors: Addario, Concetta, Milanesi, Gloria, Vaccarone, Rita, Barni, Sergio, Gerzeli, Giuseppe, Fenoglio, Carla
Format: Article
Language:English
Subjects:
Atrial natriuretic peptides
Frog
Guanylate cyclase
Heart
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Summary:The expression of atrial natriuretic peptides (ANP) - pro ANP26-92 and αANP - was investigated with immunohistochemical methods in the heart of Rana kl. esculenta by comparing active and hibernating frogs. The distribution of ANP binding sites was studied by cytochemical demonstration of guanylate cyclase (GC) activity. Concurrently, an ultrastructural study and morphometric analysis of ANP granules were conducted. Both immunohistochemistry and morphometry indicated some changes in the hibernating frogs with regard to the expression of ANP and the numerical density of ANP granules compared with active ones. GC activity was mainly detected in the ventricle and showed a decreased activity in the hibernating samples. These findings demonstrate different synthesis and release of ANP between hibernating and active frogs, suggesting that the peptides play a significant role in blood volume regulation as well as in salt and water homeostasis during the annual cycle.
ISSN:1125-0003
1748-5851
DOI:10.1080/11250000409356609