A STABILITY-INDICATING HPLC METHOD FOR THE DETERMINATION OF NAPHAZOLINE AND ITS DEGRADATION PRODUCT AND METHYL PARAHYDROXYBENZOATE IN PHARMACEUTICAL PREPARATIONS

A simple, rapid, selective, accurate, and stability-indicating HPLC method for the simultaneous determination of naphazoline and methyl parahydroxybenzoate in pharmaceutical preparations was developed and validated. The method employs a BDS Hypersil C18 column (5 µm; 150 × 4 mm), a mobile phase cont...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2014-06, Vol.37 (10), p.1321-1333
Main Authors: Korodi, T., Dulavová, M., Urban, E., Kopelent-Frank, H., Lachmann, B.
Format: Article
Language:English
Subjects:
1-naphthylacetylethylenediamine
HPLC
methyl parahydroxybenzoate
naphazoline
pharmaceutical preparation
stability indicating assay
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Summary:A simple, rapid, selective, accurate, and stability-indicating HPLC method for the simultaneous determination of naphazoline and methyl parahydroxybenzoate in pharmaceutical preparations was developed and validated. The method employs a BDS Hypersil C18 column (5 µm; 150 × 4 mm), a mobile phase containing methanol −0.05 M triethylamine plus 10% methanol pH 3 with o-phosphoric acid (30:70, v/v), with a flow rate of 1 mL/min and detection at 254 nm. All the validation parameters (linearity, specificity, accuracy, repeatability, LOD/LOQ) were within the acceptance range and met the ICH Q2(R1) guideline. The stability indicating capability of this method has been demonstrated by separating degradation products as 1-naphthylacetylethylenediamine (impurity A) and 1-naphthylacetic acid (impurity B). Stability tests were performed under alkaline, acidic, neutral, and oxidizing conditions, and with simulated sunlight under standardized conditions in a sun test irradiation chamber. The proposed method was applied for routine quality control showing the obtained assay results for various marketed preparations and for the in-house formulation to be in good agreement with the declared amount. Compared with the pharmacopoeial method, shorter retention times and a superior resolving capability have been shown.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2013.789802