Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility

To observe the seminal plasma proteomic composition in men with spinal cord injury orally treated with probenecid, in order to observe pathways associated with increased sperm motility. Prospective study. Miami Project to Cure Paralysis - University of Miami/Miller School of Medicine. Nine men with...

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Bibliographic Details
Published in:The journal of spinal cord medicine 2021-11, Vol.44 (6), p.966-971
Main Authors: Camargo, Mariana, Ibrahim, Emad, Aballa, Teodoro C., Cardozo, Karina H. M., Carvalho, Valdemir M., Lynne, Charles M., Brackett, Nancy L., Bertolla, Ricardo P.
Format: Article
Language:English
Subjects:
Chromatography, Liquid
Humans
Male
Probenecid
Probenecid - pharmacology
Probenecid - therapeutic use
Prospective Studies
Proteomics
Proteomics - methods
Semen - metabolism
Seminal plasma
Sperm Motility - physiology
Spermatozoa - metabolism
Spinal Cord Injuries - drug therapy
Spinal Cord Injuries - metabolism
Spinal cord injury
Tandem Mass Spectrometry
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Summary:To observe the seminal plasma proteomic composition in men with spinal cord injury orally treated with probenecid, in order to observe pathways associated with increased sperm motility. Prospective study. Miami Project to Cure Paralysis - University of Miami/Miller School of Medicine. Nine men with spinal cord injury, who agreed to participate in the study. Oral treatment with probenecid - 500 mg per day for one week, then 500 mg twice daily [1000 mg total] per day for three weeks. Semen analysis as per WHO 2010 guidelines, and seminal plasma proteomics analysis by LC-MS/MS. In total, 783 proteins were identified, of which, 17 were decreased, while 6 were increased after treatment. The results suggest a new pathway that could be treated by the decrease of biglycan after probenecid treatment. Oral treatment with probenecid is able to alter the seminal plasma proteome, in pathways that explain decreased innate immune response.
ISSN:1079-0268
2045-7723
DOI:10.1080/10790268.2020.1722937