Thermo-sensitive random poly(L-alanine-co-L-lactic acid) with no cytotoxicity by the structure-controlled synthesis for a nano-drug carrier

A random poly(L-alanine-co-L-lactic acid) (PAL) with excellent thermo-sensitivity and no cytotoxicity was synthesized by the structure-controlled polycondensation from natural L-alanine and L-lactic acid. Only those PALs in which the contents of L-alanine structural unit are rigidly controlled in th...

Full description

Saved in:
Bibliographic Details
Published in:International journal of polymer analysis & characterization 2017-07, Vol.22 (5), p.435-446
Main Authors: Yue, Ying, Zhao, Man, Xu, Junlian, Yang, Zhen, Zheng, Chang, Fan, Zhihui, Cao, Lili, Zhang, Miao, Deng, Kuilin
Format: Article
Language:English
Subjects:
Acids
Alanine
Cytotoxicity
Drug carrier
Drug delivery systems
L-alanine
L-lactic acid
Lactic acid
Nanostructure
no cytotoxicity
Scanning microscopy
Segments
Self-assembly
Sensitivity
thermo-sensitive polymer
Toxicity
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A random poly(L-alanine-co-L-lactic acid) (PAL) with excellent thermo-sensitivity and no cytotoxicity was synthesized by the structure-controlled polycondensation from natural L-alanine and L-lactic acid. Only those PALs in which the contents of L-alanine structural unit are rigidly controlled in the smaller range of 53-65% show a reversible lower critical solution temperature of 35-60°C. The change of secondary structure in poly(L-alanine) segments by the introduction of L-lactic acid structural unit plays a decisive role in regulating the thermo-sensitivity of PAL. The viability of HeLa cells exposed to PAL reaches up to 91-116% after incubation of 24 and 72 h, indicating no cytotoxicity. Furthermore, PAL can easily form curcumin-loaded nano-carriers through its thermo-sensitivity and self-assembly. The curcumin-loaded PAL nano-particles are observed to clearly internalize into the cells by confocal laser-scanning microscopy, and thus can be used as a potential nano-drug-carrier.
ISSN:1023-666X
1563-5341
DOI:10.1080/1023666X.2017.1315480