In vitro Antioxidant, Antityrosinase, Antibacterial and Cytotoxicity Activities of the Leaf and Stem Essential Oil from Piper magnibaccum C. DC

The chemical composition of essential oils obtained from two parts of Piper magnibaccum C.DC. were analyzed by gas chromatography with a flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). 25 components representing 93.5% of the essential oil were characterized from...

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Bibliographic Details
Published in:Journal of essential oil-bearing plants (Dehra Dun) 2017-01, Vol.20 (1), p.223-232
Main Authors: Hashim, Nur Athirah, Ahmad, Farediah, Jani, Nor Akmalazura, Susanti, Deny
Format: Article
Language:English
Subjects:
Antibacteria
Antioxidant
Antityrosinase
Cytotoxic
Essential oil
Piper magnibaccum
Piperaceae
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Summary:The chemical composition of essential oils obtained from two parts of Piper magnibaccum C.DC. were analyzed by gas chromatography with a flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). 25 components representing 93.5% of the essential oil were characterized from the leaf of P. magnibaccum. The essential oil was dominated by germacrene D (40.8%), β-caryophyllene (8.5%) and α-cadinol (6.1%). A total of 33 components, representing 87.6% of the total oil were identified from the stem of P. magnibaccum. The major constituents were β-caryophyllene (19.7%), germacrene D (10.7%), and α- cadinol (8.2%). The antioxidant potential of the leaf and stem essential oils of P. magnibaccum were determined on the basis of phenolic content and scavenging activity of the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC 50 20.5, 17.5 μg/mL) and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) (IC 50 11.7, 12.9 μg/ mL) free radicals. Antityrosinase activity of the leaf and stem were also screened using mushroom tyrosinase assay (I %= 49.50±0.6, 57.01±0.8). The essential oil of P. magnibaccum leaf showed a moderate antibacterial activity with MIC value of 250 μg/mL against P. aeruginosa. None of the essential oils exhibited acute cytotoxicity against MCF-7 and A-549 cells proliferation.
ISSN:0972-060X
0976-5026
DOI:10.1080/0972060X.2017.1282839