Release of prostaglandin D_2 by murine mast cells: importance of metabolite formation for antiproliferative activity

PROSTAGLANDIN (PG) D_2 , PGJ_2 and Δ^(12) -PGJ_2 are antiproliferative eicosanoids. We investigated the production of PGD_2 by murine bone marrow-derived mast cells (BMMC) taking into consideration metabolism of PGD_2 to PGD_2 and Δ^(12)-PGJ_2. PG-metabolites were quantified by high performance liqu...

Full description

Saved in:
Bibliographic Details
Published in:Mediators of Inflammation 1998-01, Vol.1998 (2), p.79-84
Main Authors: C. Haberl, L. Hültner, A. Flügel, M. Falk, S. Geuenich, W. Wilmanns, C. Denzlinger
Format: Article
Language:English
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:PROSTAGLANDIN (PG) D_2 , PGJ_2 and Δ^(12) -PGJ_2 are antiproliferative eicosanoids. We investigated the production of PGD_2 by murine bone marrow-derived mast cells (BMMC) taking into consideration metabolism of PGD_2 to PGD_2 and Δ^(12)-PGJ_2. PG-metabolites were quantified by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). Stimulated with calcium ionophore A23187 BMMC released eight-fold more PGJ_2 and Δ^(12)-PGJ_2 than PGD_2. Conversion of endogenously produced PGD_2 to PGJ_2 and Δ^(12)-PGJ_2 proceeded rapidly in contrast to metabolism of exogenously added PGD_2. The antiproliferative potency of these prostaglandins is demonstrated in vitro. We conclude that determination of PGD_2 production by mast cells must take into consideration rapid conversion to active derivatives, which may play a significant role in growth regulation.
ISSN:0962-9351
1466-1861
DOI:10.1080/09629359891216