Serological Determination of Nitrobacter Species in a Deodorizing Granular Activated Carbon Filter

Immunofluorescence is used to detect, to study species diversity and to count the nitrifying bacteria in a deodorization system for air polluted by ammonia. This process consists of the elimination of ammonia by a biological filter packed with granular activated carbon seeded with activated sludge e...

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Bibliographic Details
Published in:Environmental technology 1997-03, Vol.18 (3), p.275-283
Main Authors: Malhautier, L., Degorce-Dumas, J.-R., Degrange, V., Bardin, R., Le Cloirec, P.
Format: Article
Language:English
Subjects:
activated carbon
Ammonia
biofiltration
Biological and medical sciences
Biological treatment of gaseous effluents
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
count
Environment and pollution
Fundamental and applied biological sciences. Psychology
immunofluorescence
Industrial applications and implications. Economical aspects
Isolation and description
Mission oriented research
nitrifying bacteria
Nitrobacter
serology
serotypes
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Summary:Immunofluorescence is used to detect, to study species diversity and to count the nitrifying bacteria in a deodorization system for air polluted by ammonia. This process consists of the elimination of ammonia by a biological filter packed with granular activated carbon seeded with activated sludge enriched in nitrifying bacteria. Four serotypes are used, to treat Nitrobacter strains Ag, LL, W and X14. The serotypes are distributed in the same proportions throughout the filter, with the total bacteria count more concentrated near the gas source. This procedure is attractive but the count obtained is incomplete because there is no serotype for the Nitrobacter genera nor correlation between serotypes and genomic groups. Only serotypes prepared against isolated strains are known. It would therefore be interesting in a future study to isolate strains which have already colonized granular activated carbon for use in diversity studies. Another interesting avenue would be a total count of all Nitrobacter bacteria present.
ISSN:0959-3330
1479-487X
DOI:10.1080/09593331808616537