Production of a chloramphenicol-resistant mutant of Lysinibacillus sphaericus by solid state fermentation

A highly active mosquitocidal mutant of Lysinibacillus sphaericus Ahmed 2362, namely, UCR-146, was efficiently produced on cottonseed meal (CSM) medium, using sand as a carrier under solid state fermentation (SSF). The optimum CSM concentration for the highest sporulation and toxin formation was 12%...

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Bibliographic Details
Published in:Biocontrol science and technology 2013-05, Vol.23 (5), p.535-544
Main Authors: Foda, M.S., Moharam, Maysa E., El-Bendary, Magda A.
Format: Article
Language:English
Subjects:
cottonseed meal
Culex pipiens
Lysinibacillus sphaericus mutant
solid state fermentation
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Summary:A highly active mosquitocidal mutant of Lysinibacillus sphaericus Ahmed 2362, namely, UCR-146, was efficiently produced on cottonseed meal (CSM) medium, using sand as a carrier under solid state fermentation (SSF). The optimum CSM concentration for the highest sporulation and toxin formation was 12%. The maximum toxicity of the tested organism against second instar larvae of Culex pipiens was obtained at 25% moisture content, initial pH 6-7, 1% sodium acetate, 18.9×10 6 CFU/g inoculum and 6 days incubation period at 30°C. Pilot scale production of UCR-146 under the optimum SSF conditions was assessed in aluminium trays. Spore count, mortality of larvae and LC 50 of the final product were 5.5×10 10 CFU/g, 72% at 1 part per million (PPM) and 0.54 PPM, respectively. These results were comparable with those obtained from bench-scale production (in flasks). The cost of 1 kg of this bio-larvicide was estimated at US $0.34.
ISSN:0958-3157
1360-0478
DOI:10.1080/09583157.2013.790342