Ketamine/xylazine anaesthesia in the chronobiological studies

Administration of anaesthesia during the daytime is important and may influence specific aspects of in vivo animal experiments. The aim of the present study was to refer to the suitability of the use of ketamine/xylazine anaesthesia in chronobiological studies. Study was performed in ketamine-/xylaz...

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Bibliographic Details
Published in:Biological rhythm research 2014-07, Vol.45 (4), p.633-642
Main Authors: Svorc, P, Novakova, M, Bacova, I, Jurasova, Z, Marossy, A
Format: Article
Language:English
Subjects:
acid-base balance
anesthesia
animal experimentation
autonomic nervous system
bicarbonates
biological rhythms
calcium
chronobiology
electrophysiology of the heart
females
heart rate
ions
ketamine
ketamine/xylazine
rats
sodium
temperature
xylazine
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Summary:Administration of anaesthesia during the daytime is important and may influence specific aspects of in vivo animal experiments. The aim of the present study was to refer to the suitability of the use of ketamine/xylazine anaesthesia in chronobiological studies. Study was performed in ketamine-/xylazine-anaesthetized female Wistar rats (100 mg/kg/15 mg/kg, i.m) after adaptation to a light–dark (LD) cycle (12 h:12 h). Heart rate (HR), rectal temperature, ECG parameters, autonomic nervous system activity, acid-base balance and Na ⁺, K ⁺, Ca ²⁺ and Cl ⁻ plasma concentrations were evaluated in dependence on LD cycle. LD differences were found in the HR, rectal temperature, RR, PQ and QTc intervals, QRS complex, P wave amplitude, in power HF, LF and VLF components, in paO ₂ and HCO ₃⁻, and in Na ⁺, Cl ⁻ and Ca ²⁺ ions. Ketamine/xylazine anaesthesia may be applicable in chronobiological studies. It does not disturb LD differences, but it can modify them probably without loss of rhythmicity.
ISSN:1744-4179
0929-1016
1744-4179
DOI:10.1080/09291016.2014.884305