Development of a novel anti-biofilm peptide derived from profilin of Spodoptera frugiperda

Candida yeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide-PEP-IA18-was evaluated against Candida species. PEP-IA18 was designed...

Full description

Saved in:
Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2020-05, Vol.36 (5), p.516-527
Main Authors: da Silva, Amanda Carolina Borges, Sardi, Janaina de Cassia Orlandi, de Oliveira, Daniella Gorete Lourenço, de Oliveira, Caio Fernando Ramalho, dos Santos, Helder Freitas, dos Santos, Edson Lucas, Crusca, Edson, Cardoso, Marlon Henrique, Franco, Octávio Luiz, Macedo, Maria Lígia Rodrigues
Format: Article
Language:English
Subjects:
Amino acid sequence
Animals
Antifungal
Antifungal activity
Antifungal Agents - pharmacology
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antimicrobial peptides
Bioactive compounds
biofilm
Biofilms
Candida
Candida albicans
Candida spp
Cell membranes
Disease resistance
Ergosterol
Fibroblasts
Fungicides
Humans
Infections
Microbial Sensitivity Tests
Minimum inhibitory concentration
peptide
Peptides
Profilin
Profilins - pharmacology
Spodoptera - microbiology
Spodoptera frugiperda
Toxicity
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Candida yeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide-PEP-IA18-was evaluated against Candida species. PEP-IA18 was designed from the primary sequence of profilin, a protein from Spodoptera frugiperda, and displayed potent activity against Candida albicans and Candida tropicalis, showing a minimum inhibitory concentration (MIC) of 2.5 µM. Furthermore, the mechanism of action of PEP-IA18 involved interaction with the cell membrane (ergosterol complexation). Treatment at MIC and/or 10 × MIC significantly reduced biofilm formation and viability. PEP-IA18 showed low toxicity toward human fibroblasts and only revealed hemolytic activity at high concentrations. Thus, PEP-IA18 exhibited antifungal and anti-biofilm properties with potential applicability in the treatment of infections caused by Candida species.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2020.1776857