Purification and partial amino acid sequence analysis of the larval settlement-inducing pheromone from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite)

A previously undescribed larval settlement-inducing protein was purified from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite). Results of SDS-PAGE indicated that the relative molecular mass of the protein in reduced and denatured form is 31,600 ± 500 kDa, and that it is...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2009-07, Vol.25 (5), p.429-434
Main Authors: Endo, Noriyuki, Nogata, Yasuyuki, Yoshimura, Erina, Matsumura, Kiyotaka
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amphitrite
Animals
Balanus amphitrite
barnacle
Behavior, Animal
Cypris
cypris larvae
Electrophoresis, Polyacrylamide Gel
Larva - growth & development
larval settlement-inducing pheromone
Marine
Molecular Sequence Data
Molecular Weight
Pheromones - chemistry
Pheromones - isolation & purification
Thoracica - growth & development
Thoracica - metabolism
waterborne cue
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Summary:A previously undescribed larval settlement-inducing protein was purified from adult extracts of the barnacle, Balanus amphitrite (=Amphibalanus amphitrite). Results of SDS-PAGE indicated that the relative molecular mass of the protein in reduced and denatured form is 31,600 ± 500 kDa, and that it is distinct from the Settlement Inducing Protein Complex (SIPC) which has previously been determined as a larval settlement-inducing pheromone. The N-terminal 33-residue sequence of the intact protein showed no similarity with previously reported proteins in the EMBL/Genbank/DDBJ databases. The purified protein at a concentration of 10 μg ml −1 induced approximately four times more larval settlement than the control (filtered natural seawater). In addition, results of the assay using both 24-well polystyrene plates and agarose gels indicated that this protein is probably released into seawater and attracts cypris larvae. These results suggest that the purified protein is a waterborne type pheromone which induces settlement of larvae of B. amphitrite.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927010902875113