Levels of polyandry and intracolonial genetic relationships in Apis koschevnikovi

Adults of five Apis koschevnikovi colonies from Sabah, Malaysia (Borneo) were collected into alcohol and within two days, transferred to liquid nitrogen. DNA was extracted, amplified with four, or in one case six, microsatellite primer pairs or primers for a sequence tagged site, and genotypes for t...

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Bibliographic Details
Published in:Journal of apicultural research 1998-01, Vol.37 (4), p.281-287
Main Authors: Rinderer, Thomas E, Stelzer, J Anthony, Oldroyd, Benjamin P, Tingek, Salim
Format: Article
Language:English
Subjects:
Apis koschevnikovi
Borneo
DNA
genetic variation
Malaysia
microsatellite primers
multiple mating
polyandry
polymerase chain reaction
relatedness
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Summary:Adults of five Apis koschevnikovi colonies from Sabah, Malaysia (Borneo) were collected into alcohol and within two days, transferred to liquid nitrogen. DNA was extracted, amplified with four, or in one case six, microsatellite primer pairs or primers for a sequence tagged site, and genotypes for these loci were determined for 40 to 100 workers per colony. Four colonies were monogynous, one colony had worker daughters of two, probably highly related, queens. For the monogynous colonies, the queen genotypes and the number and genotypes of males mated by each queen was inferred from the worker genotypes. The queens mated with 10 to 32 drones. The average number of observed matings was 16.3 ± 10.5 and the average number of effective matings was 10.5 ± 8.4. The within-colony genetic relatedness was 0.31 ± 0.03. The fitness advantages of genetic variance are discussed.
ISSN:0021-8839
2078-6913
DOI:10.1080/00218839.1998.11100984