Determination of Sodium Hexametaphosphate in Beverages Based on the Fluorescence Quenching of Acridine Orange

Sodium hexametaphosphate was shown to form a complex with acridine orange by electrostatic interactions that induce fluorescence quenching. Analysis of fluorescence intensity showed that the process was dominated by static quenching, which was confirmed by absorption spectra and lifetime of the exci...

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Bibliographic Details
Published in:Analytical letters 2014-11, Vol.47 (16), p.2740-2746
Main Authors: Zhao, Heng Xin, Luo, Ming, Mo, Li Xin, Cheng, Liang, Ma, Zheng, Zhang, Shi Lei
Format: Article
Language:English
Subjects:
Acridine orange
Fluorescence quenching
Quantitative detection
Sodium hexametaphosphate
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Summary:Sodium hexametaphosphate was shown to form a complex with acridine orange by electrostatic interactions that induce fluorescence quenching. Analysis of fluorescence intensity showed that the process was dominated by static quenching, which was confirmed by absorption spectra and lifetime of the excited state. The decreased fluorescence intensity was directly proportional to the concentration of sodium hexametaphosphate between 8.0 × 10 −7 and 1.1 × 10 −5  mol L −1 with a limit of detection of 5.3 × 10 −7  mol L −1 . Beverages were analyzed for sodium hexametaphosphate with recoveries between 91.7% and 108.3%.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719.2014.919509