Loading…

Quantification of Carcinoembryonic Antigen by Capillary Electrophoresis-Chemiluminescence Detection using Internal Standard Method

Sensitive determination of tumor marker carcinoembryonic antigen (CEA) by capillary electrophoresis-chemiluminescence detection (CE-CL) has been developed using an internal standard method. Parameters affecting the CE separation and CL detection were systematically optimized. Under the optimal condi...

Full description

Saved in:
Bibliographic Details
Published in:Analytical letters 2009-01, Vol.42 (1), p.45-57
Main Authors: Liu, Yanming, Yue, Haiyan, Tian, Wei, Chen, Yonghong, Li, Furong
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sensitive determination of tumor marker carcinoembryonic antigen (CEA) by capillary electrophoresis-chemiluminescence detection (CE-CL) has been developed using an internal standard method. Parameters affecting the CE separation and CL detection were systematically optimized. Under the optimal conditions, the free horseradish peroxidase (HRP)-labeled antibody (Ab*), the bound Ab*-antigen complex (Ab*-Ag), and HRP used as internal standard were well separated within 5 min. The proposed method was successfully applied for the quantification of CEA in human sera obtained from both healthy persons and patients.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710802568812