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Quantification of Carcinoembryonic Antigen by Capillary Electrophoresis-Chemiluminescence Detection using Internal Standard Method
Sensitive determination of tumor marker carcinoembryonic antigen (CEA) by capillary electrophoresis-chemiluminescence detection (CE-CL) has been developed using an internal standard method. Parameters affecting the CE separation and CL detection were systematically optimized. Under the optimal condi...
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Published in: | Analytical letters 2009-01, Vol.42 (1), p.45-57 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Sensitive determination of tumor marker carcinoembryonic antigen (CEA) by capillary electrophoresis-chemiluminescence detection (CE-CL) has been developed using an internal standard method. Parameters affecting the CE separation and CL detection were systematically optimized. Under the optimal conditions, the free horseradish peroxidase (HRP)-labeled antibody (Ab*), the bound Ab*-antigen complex (Ab*-Ag), and HRP used as internal standard were well separated within 5 min. The proposed method was successfully applied for the quantification of CEA in human sera obtained from both healthy persons and patients. |
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ISSN: | 0003-2719 1532-236X |
DOI: | 10.1080/00032710802568812 |