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Cyanobacterial conversion of carbon dioxide to 2,3-butanediol
Conversion of CO₂ for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 2013-01, Vol.110 (4), p.1249-1254 |
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| creator | Oliver, John W. K. Machado, Iara M. P. Yoneda, Hisanari Atsumi, Shota |
| description | Conversion of CO₂ for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically developed the 2,3-butanediol (23BD) biosynthetic pathway in Synechococcus elongatus PCC7942 as a model system to establish design methods for efficient exogenous chemical production in cyanobacteria. We identified 23BD as a target chemical with low host toxicity, and designed an oxygen-insensitive, cofactor-matched biosynthetic pathway coupled with irreversible enzymatic steps to create a driving force toward the target. Production of 23BD from CO₂ reached 2.38 g/L, which is a significant increase for chemical production from exogenous pathways in cyanobacteria. This work demonstrates that developing strong design methods can continue to increase chemical production in cyanobacteria. |
| doi_str_mv | 10.1073/pnas.1213024110 |
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K.</creatorcontrib><creatorcontrib>Machado, Iara M. P.</creatorcontrib><creatorcontrib>Yoneda, Hisanari</creatorcontrib><creatorcontrib>Atsumi, Shota</creatorcontrib><title>Cyanobacterial conversion of carbon dioxide to 2,3-butanediol</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Conversion of CO₂ for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically developed the 2,3-butanediol (23BD) biosynthetic pathway in Synechococcus elongatus PCC7942 as a model system to establish design methods for efficient exogenous chemical production in cyanobacteria. We identified 23BD as a target chemical with low host toxicity, and designed an oxygen-insensitive, cofactor-matched biosynthetic pathway coupled with irreversible enzymatic steps to create a driving force toward the target. Production of 23BD from CO₂ reached 2.38 g/L, which is a significant increase for chemical production from exogenous pathways in cyanobacteria. This work demonstrates that developing strong design methods can continue to increase chemical production in cyanobacteria.</description><subject>Acetoin - metabolism</subject><subject>Acetolactate Synthase - genetics</subject><subject>Acetolactate Synthase - metabolism</subject><subject>Alcohol Dehydrogenase - genetics</subject><subject>Alcohol Dehydrogenase - metabolism</subject><subject>Bacteria</subject><subject>Biochemical pathways</subject><subject>Biochemistry</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biological production</subject><subject>Biological Sciences</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways</subject><subject>Butylene Glycols - metabolism</subject><subject>Carbon dioxide</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carboxy-Lyases - genetics</subject><subject>Carboxy-Lyases - metabolism</subject><subject>Chemical hazards</subject><subject>Chemical products</subject><subject>Chemicals</subject><subject>Cyanobacteria</subject><subject>Enzymes</subject><subject>Genetic engineering</subject><subject>Metabolic Engineering</subject><subject>Models, Biological</subject><subject>Photosynthesis</subject><subject>Plasmids - genetics</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Renewable Energy</subject><subject>Synechococcus - enzymology</subject><subject>Synechococcus - genetics</subject><subject>Synechococcus - metabolism</subject><subject>Synthetic Biology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpdkUlLxDAYhoMoOi5nT0rBiwer-bJMkoOCDG4geNFzSNJUO3SaMWlF_70ZRsfllO35HvLyIrQP-BSwoGfzzqRTIEAxYQB4DY0AKyjHTOF1NMKYiFIywrbQdkpTjLHiEm-iLUKJEoTwETqffJguWON6HxvTFi50bz6mJnRFqAtnos27qgnvTeWLPhTkhJZ26E3n82W7izZq0ya_97XuoKfrq8fJbXn_cHM3ubwvHaekLw0wAeCopbWvuaWGKkrGyhlh61qysXO191VlPRg7zllAynxSFSUcpJeG7qCLpXc-2JmvnO_6aFo9j83MxA8dTKP_vnTNi34Ob5pyLrAiWXD8JYjhdfCp17MmOd-2OUgYkgYiqATCMc7o0T90GobY5XgLSkhOgfFMnS0pF0NK0derzwDWi2r0ohr9U02eOPydYcV_d_ELWEyudNnHsoepDBwsgWnqQ1wRDJQCwRX9BFGXniU</recordid><startdate>20130122</startdate><enddate>20130122</enddate><creator>Oliver, John W. 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P.</au><au>Yoneda, Hisanari</au><au>Atsumi, Shota</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyanobacterial conversion of carbon dioxide to 2,3-butanediol</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2013-01-22</date><risdate>2013</risdate><volume>110</volume><issue>4</issue><spage>1249</spage><epage>1254</epage><pages>1249-1254</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>1J.W.K.O. and I.M.P.M. contributed equally to this work.</notes><notes>Edited by Robert Haselkorn, University of Chicago, Chicago, IL, and approved December 5, 2012 (received for review July 27, 2012)</notes><notes>Author contributions: J.W.K.O., I.M.P.M., and S.A. designed research; J.W.K.O., I.M.P.M., H.Y., and S.A. performed research; J.W.K.O., I.M.P.M., H.Y., and S.A. analyzed data; and J.W.K.O., I.M.P.M., and S.A. wrote the paper.</notes><abstract>Conversion of CO₂ for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically developed the 2,3-butanediol (23BD) biosynthetic pathway in Synechococcus elongatus PCC7942 as a model system to establish design methods for efficient exogenous chemical production in cyanobacteria. We identified 23BD as a target chemical with low host toxicity, and designed an oxygen-insensitive, cofactor-matched biosynthetic pathway coupled with irreversible enzymatic steps to create a driving force toward the target. Production of 23BD from CO₂ reached 2.38 g/L, which is a significant increase for chemical production from exogenous pathways in cyanobacteria. This work demonstrates that developing strong design methods can continue to increase chemical production in cyanobacteria.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>23297225</pmid><doi>10.1073/pnas.1213024110</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central; JSTOR |
| subjects | Acetoin - metabolism Acetolactate Synthase - genetics Acetolactate Synthase - metabolism Alcohol Dehydrogenase - genetics Alcohol Dehydrogenase - metabolism Bacteria Biochemical pathways Biochemistry Biodiesel fuels Biofuels Biological production Biological Sciences Biosynthesis Biosynthetic Pathways Butylene Glycols - metabolism Carbon dioxide Carbon Dioxide - metabolism Carboxy-Lyases - genetics Carboxy-Lyases - metabolism Chemical hazards Chemical products Chemicals Cyanobacteria Enzymes Genetic engineering Metabolic Engineering Models, Biological Photosynthesis Plasmids - genetics Recombinant Proteins - genetics Recombinant Proteins - metabolism Renewable Energy Synechococcus - enzymology Synechococcus - genetics Synechococcus - metabolism Synthetic Biology |
| title | Cyanobacterial conversion of carbon dioxide to 2,3-butanediol |
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