Cyanobacterial conversion of carbon dioxide to 2,3-butanediol

Conversion of CO₂ for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2013-01, Vol.110 (4), p.1249-1254
Main Authors: Oliver, John W. K., Machado, Iara M. P., Yoneda, Hisanari, Atsumi, Shota
Format: Article
Language:English
Subjects:
Acetoin - metabolism
Acetolactate Synthase - genetics
Acetolactate Synthase - metabolism
Alcohol Dehydrogenase - genetics
Alcohol Dehydrogenase - metabolism
Bacteria
Biochemical pathways
Biochemistry
Biodiesel fuels
Biofuels
Biological production
Biological Sciences
Biosynthesis
Biosynthetic Pathways
Butylene Glycols - metabolism
Carbon dioxide
Carbon Dioxide - metabolism
Carboxy-Lyases - genetics
Carboxy-Lyases - metabolism
Chemical hazards
Chemical products
Chemicals
Cyanobacteria
Enzymes
Genetic engineering
Metabolic Engineering
Models, Biological
Photosynthesis
Plasmids - genetics
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Renewable Energy
Synechococcus - enzymology
Synechococcus - genetics
Synechococcus - metabolism
Synthetic Biology
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Summary:Conversion of CO₂ for the synthesis of chemicals by photosynthetic organisms is an attractive target for establishing independence from fossil reserves. However, synthetic pathway construction in cyanobacteria is still in its infancy compared with model fermentative organisms. Here we systematically developed the 2,3-butanediol (23BD) biosynthetic pathway in Synechococcus elongatus PCC7942 as a model system to establish design methods for efficient exogenous chemical production in cyanobacteria. We identified 23BD as a target chemical with low host toxicity, and designed an oxygen-insensitive, cofactor-matched biosynthetic pathway coupled with irreversible enzymatic steps to create a driving force toward the target. Production of 23BD from CO₂ reached 2.38 g/L, which is a significant increase for chemical production from exogenous pathways in cyanobacteria. This work demonstrates that developing strong design methods can continue to increase chemical production in cyanobacteria.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1213024110