Meiotic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation

Meiotic recombination-related DNA synthesis (MRDS) was analyzed in Saccharomyces cerevisiae by specifically timed incorporation of thymidine analogs into chromosomes. Lengths and positions of incorporation tracts were determined relative to a known recombination hot spot along DNA, as was the timing...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-04, Vol.104 (14), p.5965-5970
Main Authors: Terasawa, Masahiro, Ogawa, Hideyuki, Tsukamoto, Yasumasa, Shinohara, Miki, Shirahige, Katsuhiko, Kleckner, Nancy, Ogawa, Tomoko
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biochemistry
Biological Sciences
Cell division
Chemical compounds
Chemical synthesis
Chromosomes
Chromosomes, Fungal - genetics
Chromosomes, Fungal - metabolism
Crossing Over, Genetic
Deoxyribonucleic acid
DNA
DNA probes
DNA Replication
DNA, Fungal - genetics
DNA, Fungal - metabolism
Drosophila
Drosophila - genetics
Flowers & plants
Genetic recombination
Genomics
Insects
Meiosis
Mice
Models, Genetic
Molecules
Pachytene stage
Polymerase chain reaction
Rad51 Recombinase - genetics
Rec A Recombinases - genetics
Recombination, Genetic
Research universities
Rodents
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - genetics
Synaptonemal Complex - genetics
Yeast
Yeasts
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Summary:Meiotic recombination-related DNA synthesis (MRDS) was analyzed in Saccharomyces cerevisiae by specifically timed incorporation of thymidine analogs into chromosomes. Lengths and positions of incorporation tracts were determined relative to a known recombination hot spot along DNA, as was the timing and localization of incorporation relative to forming and formed synaptonemal complex in spread chromosomes. Distinct patterns could be specifically associated with the majority cross-over and non-cross-over recombination processes. The results obtained provide direct evidence for key aspects of current consensus recombination models, provide information regarding temporal and spatial relationships between non-cross-over formation and the synaptonemal complex, and raise the possibility that removal of RecA homolog Rad51 plays a key role in regulating onset of MRDS. Finally, classical observations on MRDS in Drosophila, mouse, and lily are readily mapped onto the findings presented here, providing further evidence for a broadly conserved meiotic recombination process.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0611490104