Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach

Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic r...

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Bibliographic Details
Published in:Applied physics letters 2021-05, Vol.118 (18), p.184102-184102
Main Authors: Kim, Sangnam, Moon, Sunho, Rho, Sunghoon, Yoon, Sangpil
Format: Article
Language:English
Subjects:
Acoustic measurement
Acoustics
Applied physics
Energy transfer
Fluorescence
Gelatin
Interdisciplinary Applied Physics
Pulse duration
Sound waves
Transducers
Ultrahigh frequencies
Ultrasonic transducers
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Summary:Even though ultrahigh frequency ultrasonic transducers over 60 MHz have been used for single-cell-level manipulation such as intracellular delivery, acoustic tweezers, and stimulation to investigate cell phenotype and cell mechanics, no techniques have been available to measure the actual acoustic radiation force (ARF) applied to target cells. Therefore, we have developed an approach to measure the ARF of ultrahigh frequency ultrasonic transducers using a theoretical model of the dynamics of a solid sphere in a gelatin phantom. To estimate ARF at the focus of a 130 MHz transducer, we matched measured maximum displacements of a solid sphere with theoretical calculations. We selected appropriate ranges of input voltages and pulse durations for single-cell applications, and the estimated ARF was in the range of tens of μN. To gauge the influence of pulse duration, an impulse of different pulse durations was estimated. Fluorescence resonance energy transfer live cell imaging was demonstrated to visualize calcium transport between cells after a target single cell was stimulated by the developed ultrasonic transducer.
ISSN:0003-6951
1077-3118
DOI:10.1063/5.0044512