Ordered stretching of single molecules of deoxyribose nucleic acid between microfabricated polystyrene lines

A technique for creating arrays of parallel, stretched single molecules of deoxyribose nucleic acid (DNA) on an arbitrary substrate for high-resolution scanning-probe imaging is discussed. The technique consists of lithographically patterning polystyrene lines on a substrate which then provide attac...

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Bibliographic Details
Published in:Applied physics letters 2001-04, Vol.78 (16), p.2396-2398
Main Authors: Klein, D. C. G., Gurevich, L., Janssen, J. W., Kouwenhoven, L. P., Carbeck, J. D., Sohn, L. L.
Format: Article
Language:English
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Summary:A technique for creating arrays of parallel, stretched single molecules of deoxyribose nucleic acid (DNA) on an arbitrary substrate for high-resolution scanning-probe imaging is discussed. The technique consists of lithographically patterning polystyrene lines on a substrate which then provide attachment sites for the ends of individual DNA molecules. Molecular combing is performed to stretch DNA from one polystyrene line to the other. Scanning-tunneling and atomic-force microscope images of single molecules of bacteriophage-lambda DNA are shown to demonstrate the advantages of this technique. Several applications, from high-resolution genomics to molecular electronics, are discussed.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.1365099