Assessment of collagen content in fish skin - development of a flow analysis method for hydroxyproline determination

This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammali...

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Published in:Analytical methods 2023-11, Vol.15 (43), p.591-598
Main Authors: Melo, Maria M. P, Mesquita, Raquel B. R, Coscueta, Ezequiel R, Pintado, Manuela E, Rangel, António O. S. S
Format: Article
Language:English
Subjects:
Amino acids
Collagen
Error analysis
Fish skins
Hydroxyproline
Nutritive value
Oxidation
Skin
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description This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1 , with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1 . Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error < 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD < 1%). Flow injection method for the quantification of hydroxyproline to corelate with collagen content in blue shark skin.
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P ; Mesquita, Raquel B. R ; Coscueta, Ezequiel R ; Pintado, Manuela E ; Rangel, António O. S. S</creator><creatorcontrib>Melo, Maria M. P ; Mesquita, Raquel B. R ; Coscueta, Ezequiel R ; Pintado, Manuela E ; Rangel, António O. S. S</creatorcontrib><description>This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1 , with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1 . Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error &lt; 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD &lt; 1%). 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S</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Melo, Maria M. P</au><au>Mesquita, Raquel B. R</au><au>Coscueta, Ezequiel R</au><au>Pintado, Manuela E</au><au>Rangel, António O. S. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of collagen content in fish skin - development of a flow analysis method for hydroxyproline determination</atitle><jtitle>Analytical methods</jtitle><date>2023-11-09</date><risdate>2023</risdate><volume>15</volume><issue>43</issue><spage>591</spage><epage>598</epage><pages>591-598</pages><issn>1759-9660</issn><eissn>1759-9679</eissn><notes>Maria Manuela Estevez Pintado has a BSc in Pharmacy from the Faculty of Pharmacy of the University of Porto (1991) and PhD in Biotechnology from the Portuguese Catholic University (UCP) (1999). She is Associate Professor, Director of CBQF - State Associate Laboratory and Associate Director of the College of Biotechnology of UCP. She is the leader of the Biobased and Biomedical products group and coordinates the Bioactives and Bioproducts Research Laboratory. She has established strong interaction with industry mainly in the food sector. Her main research interests include development and characterization of bioactives and by-product valorization through bioprocesses and extraction processes aiming to obtain high-added value products to be applied to food and other industries. She has co-authored</notes><notes>ca.</notes><notes>90 externally funded projects.</notes><notes>Maria Melo completed, in November 2022, her MSc in Biomedical Engineering (final grade of 18/20) in Escola Superior de Biotecnologia (Universidade Católica Portuguesa) and, in the same institution, she received her BSc in Bioengineering (final grade of 17/20), in 2020. Maria is a researcher at Centro de Biotecnologia e Química Fina, after winning a research grant in the project FISHCOLBOOSTER. During the course of her master's degree, she attended an Erasmus+ program during one semester at Univesità degli studi di Verona (Italy) and had presented her work in an international conference. Throughout her academic path, she also received merit awards and participated in the organisation of conferences and other events, as well as in other dynamic activities, such as in a students' union and volunteering.</notes><notes>Raquel Beatriz Ribeiro de Mesquita completed her PhD in Biotecnologia in 2007 at Universidade Católica Portuguesa Escola Superior de Biotecnologia (UCP-ESB), bachelor's degree in Microbiologia in 1999 at UCP-ESB and master's degree (MSc) in Biotechnology in 2000 at De Monfort University. She is a contracted researcher at UCP-ESB, has published 61 articles in peer reviewed journals and has been in the organizing committee of two international conferences. She has been invited to lecture in scientific symposiums (#8) and in seminars (#3) and presented/co-authored her work in both national and international scientific conferences. She supervised 5 MSc dissertations and co-supervised 3 PhD theses. She has received over 10 awards and/or honors including a FIA Award for younger researchers by the Japanese Association for Flow Injection Analysis (JAFIA) committee and an Endeavour Research Fellowship by the Australian Government Department of Education. She works in the areas of Exact Sciences with emphasis on Chemical Sciences and Analytical Chemistry, applied to Environmental Sciences and Medical and Health Sciences.</notes><notes>400 papers in international journals, is a creator of 20 patents, has been involved in the supervision/co-supervision of 34 concluded PhD theses and has coordinated/co-coordinated</notes><notes>António Osmaro Santos Silva Rangel is Full Professor at Universidade Católica Portuguesa (ESB-UCP), Porto, Portugal. He is President of the Scientific Board of ESB and member of the Direction Board of the Research Center. He holds a degree in Chemistry (Univ. Porto, 1985) and a PhD degree in Biotechnology (UCP, 1991). He has a 35 year teaching activity involving general chemistry and analytical chemistry courses. His research interests are focused on flow-based methods for the automation of (bio)chemical assays in the environmental, food, and biomedical sectors. He has supervised 20 PhD theses and is co-author of more than 200 papers.</notes><notes>Ezequiel R. Coscueta is a junior researcher at CBQF and Invited Assistant Professor at ESB-UCP. He has a PhD in Biological Sciences (National University of Rosario, 2018) and BSc in Biotechnology (National University of the Littoral, 2013). He is a technology developer within the circular economy approach with expertise in green chemistry, peptidomics, and nanotechnology for the food and nutraceutical industries. His career consists of 8 years in Food Sciences, Biotechnological Engineering, and Nanotechnology, particularly in the isolation, purification, analysis, and delivery of bioactive compounds. He's specialized in the development of novel green technology for the valorisation of agri-food by-products, in a sustainable and efficient way. Likewise, his current interests align with the development of alternative matrices and ingredients for the next generation of food and nutraceutical products, and the resilience of food systems. He is an author of 25 international peer-reviewed articles, one book, and five chapters. He is also an editor of 4 issues in high-impact international journals and a co-author of 1 patent.</notes><notes>Electronic supplementary information (ESI) available. See DOI</notes><notes>https://doi.org/10.1039/d3ay01589k</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1 , with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1 . Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error &lt; 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD &lt; 1%). 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source Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list)
subjects Amino acids
Collagen
Error analysis
Fish skins
Hydroxyproline
Nutritive value
Oxidation
Skin
title Assessment of collagen content in fish skin - development of a flow analysis method for hydroxyproline determination
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